海绵共附生芽孢杆菌中bacillamide C的快速分离  

Fast separation of bacillamide C from a Bacillus atrophaeus strain associated with a sponge

作　　者：金靓婕[1] 梁承红[1] 李荫[1] 李志勇[2] 

机构地区：[1]海军航空工程学院基础实验部,山东烟台264001 [2]上海交通大学海洋生物技术实验室,上海200240

出　　处：《化学研究与应用》2014年第10期1623-1626,共4页Chemical Research and Application

基　　金：国家自然科学基金项目(41076077)资助

摘　　要：以一株海绵共附生芽孢杆菌发酵液的乙酸乙酯萃取物为原料,分别用硅胶吸附柱层析、离心薄层层析和半制备HPLC三种方法对其中的天然杀藻活性物质bacillamide C进行分离提纯,通过对分离过程中进样量、时间、洗脱液消耗量和分离物中bacillamide C的纯度和回收率的分析比较,对三种方法的优缺点做出综合评定,认为半制备HPLC法有成为理想的快速分离bacillamide C方法的潜力。With the EtOAC extracts of fermentation broth of a Bacillus atrophaeus strain associated with a sponge as the material,the natural algicide bacillamide C was separated and purified by three different methods：silica gel column chromatography,centrifugal TLC and semi-preparative HPLC. By comparing injection volume, time, eluent consumption, the purity and the recovery of bacil-lamide C,both advantages and disadvantages of the three methods were evaluated comprehensively. The results showed that the semi-preparative HPLC has the potential to become the ideal method for rapid separation of bacillamide C.

关 键 词：bacillamide c 柱层析 离心薄层层析 半制备HPLC 

分 类 号：O658.1[理学—分析化学]