牦牛脑组织不同部位SLC25A6 mRNA绝对定量比较研究  

Comparative study for the absolute quantification of the SLC25A6 mRNA expressions in the different parts of the brain tissue of yak

作　　者：王志强[1] 荆海霞[1] 俞红贤[1] 张勤文[1] 魏青[1] 梁林[1] 牛亮[1] 

机构地区：[1]青海大学,西宁810016

出　　处：《黑龙江畜牧兽医》2014年第10期29-31,34,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31260588);青海省科技厅项目(2011-Z-901)

摘　　要：为了研究SLC25A6 mRNA在牦牛脑组织中的表达量对于能量利用的影响,试验采用绝对定量方法,建立用于SLC25A6基因绝对定量的标准曲线。结果表明:标准曲线扩增效率(E)为104.0%,回归系数(R2)为0.998,斜率为-3.230,溶解曲线峰值单一。牦牛脑组织各部位SLC25A6mRNA表达量总体差异有统计学意义(P<0.05),SLC25A6 mRNA检测值从高至低依次为小脑、海马、枕叶、顶叶、颞叶、额叶,小脑SLC25A6 mRNA表达量显著高于其他各组织(P<0.05);额叶表达量最低(P<0.05),小脑表达量为额叶表达量的3.7倍;顶叶、枕叶、海马之间差异无统计学意义(P>0.05),额叶与颞叶之间差异无统计学意义(P>0.05)。说明牦牛脑组织中小脑耗能强度可能最大,细胞活动旺盛;相反额叶耗能强度最低。To study the effect of SLC25A6 mRNA expression levels in the brain tissue of yak on energy utilization,a standard curve for SLC25A6 genetic absolute quantification was established using the method of absolute quantification. The results showed that the amplification efficiency （E） of standard curve was 104.0%, and its regression coefficient was R2 = 0. 998, and the slope was -3. 230, the peak value of melting curve was single; the detection values of absolute quantification showed that the overall difference in the expression levels of the SLC25A6 mRNA in each parts of the brain tissue of yak ,was statistically significant（ P 〈0.05 ） ,and the detection values of the SLC25A6 mRNA ranging from high to low were in the cerebellum, hippocampus, occipital lobe, parietal lobe, temporal lobe, and frontal lobe, and the expression level of the SLC25A6 gene in the cerebellum was significantly higher than that in the other tissues（ P 〈 0.05 ） ; the lowest expression level of the SLC25A6 was detected in the frontal lobe （ P 〈 0.05 ）, and the expression level of the gene in cerebellum was 3.7 times as high as that in the frontal lobe ; the differences of the expression levels in the hippocampus, occipital lobe, parietal lobe were not statistically significant （ P 〉 0.05 ）, neither were in the frontal lobe and temporal lobe（ P 〉0.05 ）. The results indicate that the energy consumption intensity in cerebellum may be greatest in the brain tissue of yak, and the cellular activities are vigorous, while the energy consumption intensity in the frontal lobe is lowest.

关 键 词：牦牛 脑组织 线粒体 SLC25A6基因 绝对定量 

分 类 号：S823.85[农业科学—畜牧学]