奶牛乳腺肥大细胞数量与胶原纤维面积的相关性研究  

Correlational study of the number of mast cells and the area of collagen fibers in the mammary gland of the dairy cow

作　　者：杨彬[1] 杨隽[1] 徐丹丹[1] 孙志鹏[1] 朱金海[1] 武瑞[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《黑龙江畜牧兽医》2014年第10期32-34,227,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江八一农垦大学博士启动基金项目(2031011037)

摘　　要：为了研究奶牛乳腺肥大细胞的数量与奶牛乳腺胶原纤维面积的相关性,试验对奶牛泌乳期和静止期乳腺进行采样,应用甲苯胺蓝组织化学染色法观察奶牛泌乳期和静止期乳腺肥大细胞数量的变化特点,应用苦味酸天狼猩红染色法观察奶牛泌乳期和静止期乳腺胶原纤维面积的变化特点。结果表明:静止期奶牛乳腺肥大细胞的数量明显比泌乳期多(P<0.01),静止期奶牛乳腺胶原纤维的面积明显比泌乳期大(P<0.01)。静止期奶牛乳腺肥大细胞的数量多,胶原纤维的面积也大;泌乳期的奶牛乳腺肥大细胞的数量少,胶原纤维的面积也小。说明奶牛乳腺肥大细胞的数量与胶原纤维的面积存在明显正相关性(r=0.677,P<0.05)。To study the correlation between the number of mast cells and the area of collagen fibers in the mammary gland of the dairy cow, the specimens from the mammary gland of dairy cow during the lactation and non - lactation periods were collected. A toluidine blue staining method was used to observe the changes in the number and distribution of the mast cells, and a picric sirius rod staining method was used to observe the changes in the characteristics of the area and distribution of collagen fibers. The results showed that the number of mast cells during the non - lactation period was significantly more than （ P 〈 0.01 ） that during the lactation period. The area of collagen fibers during the non - lactation period was significantly larger than （P 〈 0.01 ） that during the lactation period. The number of mast cells increased, and the area of collagen fibers was also big during the non - lactation period ;the number of mast cells decreased, and the area of collagen fibers was also small during the lactation period. The results indicate that there was significant positive correlation between the number of mast cells and the area of collagen fi- bers in the mammary gland of dairy cow （r = 0. 677,P 〈 0.05 ）.

关 键 词：奶牛 乳腺 肥大细胞 胶原纤维 泌乳期 静止期 

分 类 号：S823.91[农业科学—畜牧学]