蒙古沙冬青根系深色有隔内生真菌共生及分离培养特征研究  

The symbiotic and isolated culture characteristics of DSE in roots of Ammopiptanthus mongolicus

作　　者：陈严严[1] 贺学礼[1] 李宝库[1] 程春泉[1] 姜桥[1] 陈伟燕[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北农业大学学报》2014年第5期18-24,共7页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31170488)

摘　　要：2013年7月选取宁夏银川、沙坡头和甘肃民勤3个样地,分0-10,10-20,20-30,30-40,40-50cm5个土层采集蒙古沙冬青（Ammopiptanthus mongolicus）根围土壤样品,系统研究了蒙古沙冬青根系深色有隔内生真菌（DSE）侵染率、空间分布和分离培养特征,以便阐明DSE与蒙古沙冬青共生关系,为利用DSE资源促进蒙古沙冬青生长和荒漠植被恢复提供依据。结果表明：蒙古沙冬青根系能被DSE高度侵染,并发育形成典型的有隔菌丝和微菌核结构,银川样地微菌核以离散型分布为主,聚合型少见;沙坡头和民勤样地聚合型分布比例增加;微菌核数/根段长度比值,银川和沙坡头样地差异显著;微菌核/根段面积比值,仅10-20cm土层沙坡头与银川和民勤差异显著。菌丝侵染率最大值在0-20cm土层,民勤显著低于银川和沙坡头;微菌核侵染率20-30cm土层较高;总侵染率和定殖强度最大值在0-30cm土层,同一土层民勤与银川和沙坡头差异显著。共分离10株DSE菌株,5株产孢,5株不产孢,不产孢菌株多分离自0-10cm土层,而产孢菌株多分离自20-30cm和40-50cm土层。To understand the ecological significance of dark septate endophyte（DSE）associations in arid lands,the spatial dynamic of DSE fungi were surveyed in three plots including Yinchuan,Shapotou and Minqin located in northwestern sandland,China,in July 2013.Soil samples were collected in the rhizosphere of Ammopiptanthus mongolicus and were divided into five depth intervals,0-10,10-20,20-30,30-40 and 40-50 cm,respectively.The results showed that A.mongolicus could be highly infected by DSE and form a classical type of septate hyphae and microsclerotia,most of microsclerotia was discrete distribution and polymeric type was rare in Yinchuan.The proportion of polymeric type increased in Shapotou and Minqin.The ratio of microsclerotia/root length had significant differences between Yinchuan and Shapotou.The ratio of microsclerotia/root area had significant differences at the only10-20 cm depth among Shapotou and Yinchuan and Minqin.The highest hyphal infection was at 0-20 cm depth,and that in Minqin was significant lower than in Yinchuan and Shapotou.Microsclerotia infection was higher at 20-30 cm depth.The highest total infection and intensity were at 0-30 cm depth,there was a significant difference at the same depth among Minqin and Yinchuan and Shapotou.The 10 strains of DSE were isolated from roots,5strains could sporulate and the other strains could not sporulate,more often than not the asporulated strains were isolated from 0-10 cm depth and the sporulated strains were isolated from 20-30 cm and 40-50 cm depths.

关 键 词：DSE 侵染结构 分离培养 蒙古沙冬青 荒漠环境 

分 类 号：S793.9[农业科学—林木遗传育种]