整合蛋白β1在人类卵母细胞和早期胚胎上分布的研究  

INTEGRIN β1 MAY PLAY IMPORTANT ROLE IN HUMAN EGG FERTILIZATION AND EARLY EMBRYO DEVELOPMENT:A CONFOCAL MICROSCOPY STUDY

作　　者：韩毅冰[1,3] 蔡志明[2] 孙青原[4] 陈系古[1] 

机构地区：[1]中山医科大学实验动物中心,广州510089 [2]深圳市中心医院,深圳518006 [3]深圳市罗湖区人民医院,生殖医学中心,北京100080 [4]中国科学院动物所,生殖生物学国家重点实验室

出　　处：《解剖学报》2002年第4期400-404,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金重大基础项目研究"973"课题 (G19990 5 5 90 2 );深圳市卫生局基础研究课题资助项目 ( 19990 6 0 0 3)

摘　　要：目的　研究人类卵母细胞体外成熟和体外受精前后 ,以及体外受精后胚胎早期发育的不同阶段 ,整合蛋白 β1的分布规律。 　方法　利用激光共聚焦显微镜和荧光标记的整合蛋白 β1抗体。 　结果　在成熟人类卵母细胞、体外受精的合子以及 2细胞阶段胚胎中 ,整合蛋白 β1的分布不同于在小鼠胚胎中的分布 ,不是分布在细胞质膜的表面 ,而是集中分布在细胞核的附近 ,细胞质膜的表面分布很少。在体外培养的 4细胞和 8细胞胚胎中 ,整合蛋白 β1均匀分布在卵裂球中。发育至桑椹胚阶段 ,整合蛋白 β1的分布开始出现极性 ,到囊胚阶段 ,整合蛋白 β1的分布集中于滋养外胚层处。　结论　整合蛋白分子被普遍认为是卵母细胞上的精子受体 ,但本研究提示 ,人类精子和卵母细胞的结合 ,整合蛋白 β1的影响可能不大。而整合蛋白β1可能与雌雄原核融合。Objective To investigate distribution of integrain β1 in human oocytes and embryos before and after maturation/fertilization,dring the first and second meiosis,in morulae and blastocyst stage. Methods Human oocytes and embryos were stained by anti-integrin β1 (PharMingen 09351D) and FITC-labeled second antibody (PharMingen 110094D),and observed by confocal microscope. Results Integrin β1 concentrated in the nuclear area in matured oocytes,zygotes and 2-cell stage embryo,indicating that integrin β1 might involve in mitosis.Dislike mouse,there was no distribution of integrin β1 on the well-accepted sperm binding area on the oocytes,indicating that integrin β1 might not involve in the binding and fusion of human sperm to human oocytes.Conclusion It was first postulated that integrin β1 might actively involve in the pronuclear fusion process in human in vitro fertilized oocytes.The marker of ectotrophoblasts,polarized distribution of integrin β1, appeared at the morulae stage.The stronger expression in ectotrophoblasts around the inner cell mass might indicate some specific function of the ectotrophoblasts near the ICM.

关 键 词：卵母细胞 胚胎 体外受精 整合蛋白β1 激光共聚焦显微镜 体外成熟 

分 类 号：R321－33[医药卫生—人体解剖和组织胚胎学]