中等剂量(20Gy)辐照对大鼠脑内c-fos表达的早期影响  

EARLY EFFECT OF MEDIUM-DOSE IONIC IRRADIATION (20Gy)ONTHE EXPRESSION OF c-fos IN THE RAT BRAIN

作　　者：邱勇[1] 石梅[2] 孙朝阳[2] 魏丽春[2] 段丽[1] 陈良为[1] 饶志仁[1] 

机构地区：[1]第四军医大学神经科学研究所,西安710032 [2]第四军医大学西京医院放射治疗科,西安710032

出　　处：《解剖学报》2002年第4期415-419,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 39970 2 30 )

摘　　要：目的　探讨电离辐射对大鼠脑内Fos蛋白表达的早期影响。　方法　应用大鼠半脑 2 0Gy照射模型 ,免疫组织化学方法 ,观察Fos蛋白在脑内的表达及分布情况。　结果　受照射后 2 4h、1周大鼠脑内各部位Fos蛋白表达均明显减少 ,随着时间的延长 ,其Fos免疫反应性细胞数量逐渐增加 ,照射后 4周 ,延髓、脑桥内Fos免疫阳性细胞数量恢复并超过正常对照组水平 ,但中脑、间脑及端脑内未恢复到正常对照组水平。　结论　结果提示 :中等剂量辐射后早期 ,脑内许多核团的神经细胞功能活动可能受到抑制 。Objective To investigate the early effect of medium-dose ionic irradiation on the expression of Fos protein in the rat brain. Methods Fos protein was observed in rat brains at times ranging from 24 hours to 4 weeks after hemispheric irradiation (single-fraction maximal dose of 20Gy) with the immunohistochemical technique. Results Compared with that of the un-radiated rats,the expression of Fos protein in the irradiated brain decreased distinctly 24 hours and 1 week after irradiation.However,the quantity of Fos immunopositive cells increased gradually afterwards.At four weeks after radiation,expression of Fos protein recovered progressively in medulla oblongata and pons,in which Fos immunopositive cells were more than those in control group.In contrast,expression level of Fos protein in mesencephalon,diencephalons or telencephalon was still less compared with that of the un-irradiated rats.Conclusion The result suggested that the neuronal activity might be inhibited in certain nuclei of the rat brain in early stages after hemisphere irradiation,and this inhibitory phenomenon was more obviously in higher neural centers.

关 键 词：电离辐射 FOS蛋白 脑 免疫组织化学 半脑20Gy照射模型 

分 类 号：R811.5[医药卫生—放射医学]