Changes of Spontaneous Electrical Activities of Olfactory Bulb in Bufo gargarizans during Postembryonic Development  

中华蟾蜍胚后嗅球神经元自发电活动的发育变化

作　　者：彭霞[1] 孙敏[2] 梁传成[2] 王勇[1] 王丽文[2] 

机构地区：[1]沈阳师范大学实验教学中心,辽宁沈阳110034 [2]沈阳师范大学化学与生命科学学院,辽宁沈阳110034

出　　处：《Agricultural Science & Technology》2014年第10期1751-1753,共3页农业科学与技术（英文版）

基　　金：Supported by grants from the Liaoning Provincial Department of Education(2011196);the Experimental Center Director Fund of Shenyang Normal University(sy201003)~~

摘　　要：Objective] The aim of this study was to investigate the electrophysiological characteristics of spontaneous electrical activities of olfactory bulb in Bufo gargarizans during postembryonic development. [Method] Microelectrode technique was used to record extracellular spontaneous electrical activities of olfactory bulb in B. gar-garizans. [Result] Along with the development of olfactory bulb, the discharge types increased gradual y. Single spontaneous firing enhanced gradual y and it reached the maximum in the metamorphosis phase. The intensity of consecutive single sponta-neous firing changed slightly at the middle of post-embryonic development, but it was stil higher than in the early phase and the adult phase. Consecutive burst spontaneous firing frequency went down over time. [Conclusion] Along with the de-velopment of olfactory bulb, the neuronal excitability increases step by step and the neuronal electrical activities diversify gradualy.[目的]探讨中华蟾蜍(Bufo bufo gargarizans)胚后嗅球神经元自发电活的电生理学特性的发育变化。[方法]应用微电极电生理技术,对中华蟾蜍胚后嗅球神经元的自发放电活动进行在体胞外记录。[结果]随着嗅球的发育,其神经元的放电形式逐渐增多,单个放电振幅逐渐增大,且在变态期达最大;连续放电振幅在胚后发育的中期变化不大,但高于发育的早期和成体期;连续簇状放电频率随着发育时期而降低,但在成体期再次升高,而连续单个放电频率只在成体期增大。[结论]随着嗅球的发育,神经元的兴奋性逐步提高,神经元电活动形式逐渐呈现多样化。

关 键 词：Bufo gargarizans Olfactory bulb Spontaneous electrical activities Postembryonic development 

分 类 号：Q424[生物学—神经生物学]