基于28S rDNA序列的2种飞虱内生真菌多样性研究  

Endosymbionts diversity in two planthoppers estimated by 28S rDNA analysis

作　　者：王瑶[1] 王玉龙[1] 章西 陈名君[1] 黄勃[1] 

机构地区：[1]安徽农业大学安徽省微生物防治重点实验室,合肥230036

出　　处：《安徽农业大学学报》2014年第5期768-771,共4页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(30972368)资助

摘　　要：飞虱是农业的重要害虫之一,其体内存在大量共生菌,它们为寄主提供氨基酸、固醇类物质以及蛋白质等多种营养成分,保证飞虱的正常生长和繁殖。利用对飞虱内生真菌28S rDNA基因克隆和序列比对分析的方法,首次对烟翅白背飞虱SogatellaKolophon和长绿飞虱Saccharosydneprocerus体内内生真菌的多样性进行研究。结果显示,烟翅白背飞虱腹部脂肪体内可能存在4种内生真菌,其中热带假丝酵母Candidatropicalis为优势共生菌;长绿飞虱可能存在6种内生真菌,其中担子菌酵母Derxomyces anomala和泡状莫氏黑粉菌Moesziomycesbullatus系为飞虱首次发现。另外,在这2种飞虱体内均发现了一种与白地霉Galactomyces geotrichum相似度为99%的内生真菌,这也是该菌在飞虱体内的首次报道。本研究为以后探讨内生真菌与其宿主的进化关系及探索利用内生菌防治飞虱害虫奠定了基础。The planthopper, one of the most important agricultural pests, harbors a large group of endosymbio-tes that provide amino acids and other nutrients for the growth, development, and reproduction af their host planthoppers. The diversity of endosymbiotes in Sogatella kolophon and Saccharosydne procerus was investigated basedon 28S rDNA sequence analysis. The results showed that four symbiotes were detected within abdominal fat body ofS. kolophon in which the symbiote Candida tropicalis was dominant. S. procerus contained six symbiotes in whichDerxomyces anomala and Moesziomyces bultatus were reported in planthoppers for the first time. We further identi-fied one endosymbiote that has high homology （99%） to Galactomyces geotrichum in both S. kolophon and S. pro-cerus, which is the first report in planthoppers. This research has laid a foundation for the future investigation ofco-evolution between the endophytic fungi and planthoppers and the control of planthoppers using the endophyticfungi.

关 键 词：烟翅白背飞虱 长绿飞虱 内生真菌 28S RDNA 多样性 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]