霍寿黑猪和长白猪TLR4基因第3外显子的SNP检测及其遗传差异分析  

Single nucleotide polymorphism(SNP) detection and genetic difference analysis of TLR4 gene exon 3 in Huoshou black and landrance pigs

作　　者：朱卫华[1] 杜恒[1] 杨勇 薛玮玮[1] 孟云[1] 黄龙[1] 张晓东[1] 丁月云[1] 殷宗俊[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽地方畜禽遗传资源保护与生物育种省级实验室,合肥230036 [2]安徽安泰农业集团,广德242200

出　　处：《安徽农业大学学报》2014年第5期808-812,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(31171200);安徽省科技攻关重点项目(1301031032);国家农业科技成果转化资金项目(2012GB2C300207);科技富民强县专项行动计划;安徽畜禽产业共性技术研究院共同资助

摘　　要：为了对安徽地方猪霍寿黑猪和引进猪种长白猪TLR4基因进行多态性分析,采用PCR-SSCP结合PCR产物双向测序的方法研究了86头霍寿黑猪及44头长白猪2个群体共130个样本TLR4基因外显子3部分片段的单核苷核多态性,并对检测到的突变位点进行群体遗传学分析。结果检测到1个突变C1027A,C1027A突变为错义突变。群体遗传学分析表明,该多态位点基因型的分布在霍寿黑猪和长白猪2个中外猪种间存在极显著差异(P<0.01)。C1027A位点多态性在地方猪品种和引进品种间的差异,是否与品种抗病性有关,值得进一步研究。Single nucleotide polymorphisms of TLR4 exon 3 in 86 Huoshou black and 44 landrance pigs （atotal of 130 individuals in two populations） were detected using PCR-SSCP and bi-directional DNA sequencing.The detected SNPs were further analyzed using population genetics for their genetic variations. One SNP site（C1027A） was detected to be a no-synonymous SNP site. The result showed that the distribution of theC1027A-polymorphic genotype was significantly different （P〈0.01） at the 1027 locus between Landrace and Hu-oshou black pigs. Such differences at the 1027 locus between local and introduced pigs might correlate with resis-tance of certain diseases.

关 键 词：霍寿黑猪 长白猪 TLR4基因 SNP 群体遗传学分析 

分 类 号：S813.3[农业科学—畜牧学]