3个安徽地方猪品种TAP1基因编码区遗传多态性分析  

Single nucleotide polymorphisms(SNPs) in TAP1 coding sequence in three Anhui native pig breeds

作　　者：薛玮玮[1] 王源朗 杨勇 朱卫华[1] 孟云[1] 黄龙[1] 张晓东[1] 丁月云[1] 殷宗俊[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽地方畜禽遗传资源保护与生物育种省级实验室,合肥230036 [2]安徽安泰农业集团,广德242200

出　　处：《安徽农业大学学报》2014年第5期813-817,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(31171200);安徽省科技攻关重点项目(1301031032);国家农业科技成果转化资金项目(2012GB2C300207);科技富民强县专项行动计划;安徽畜禽产业共性技术研究院项目共同资助

摘　　要：以3个安徽地方猪品种皖南黑猪、圩猪和霍寿黑猪共324头猪为研究对象,采用PCR-SSCP方法检测猪TAP1基因编码区的遗传多态性。结果表明,在皖南黑猪、圩猪和霍寿黑猪TAP1基因的第10外显子41 bp碱基处发生G>A突变,检测到AA、GA、GG 3种基因型,其中AA基因型频率在这3个群体中分别为59.53%、41.27%和54.91%,为优势基因型;皖南黑猪、圩猪和霍寿黑猪的多态信息含量(PIC)分别为0.2991、0.3611和0.3242,在该位点均处于中度多态。卡方检验表明,皖南黑猪、圩猪和霍寿黑猪群体均处于哈迪-温伯格平衡状态(P>0.05)。A total of 324 pigs from Wannan black pig, Wei pig, and Huoshou black pig populations were usedto detect polymorphisms of the TAP1 gene coding sequence using PCR-SSCP method. Results showed that a G 〉A mutation occurred at 41-base-position in exon 10, resulted in three genotypes （AA, GA and GG） in the three pigpopulations. The genotypic frequencies of AA in Wannan black pig, Wei pig, and Huoshou black pig populationwas 59.53%, 41.27%, and 54.91%, respectively. Genotype AA is a dominant genotype. The polymorphism infor-mation content （PIC） was 0.2991 in Wannan black, 0.3611 in Wei pig, and 0.3242 in Huoshou black pig popula-tion, which was ranged at the medium level of polymorphism. The Z2 test indicated the Hardy-Weinberg equilib-rium in the three pig populations （P〉0.05）.

关 键 词：安徽地方猪 TAP1基因 单核苷酸多态性 遗传分析 

分 类 号：S813.3[农业科学—畜牧学]