响应面法优化沙芥叶片总黄酮提取工艺的研究  

Optimizing the extraction process of total flavonoids from Pugionium cornutum leaves using Response Surface Methodology

作　　者：徐世才[1] 谢轶博 杨晓燕[1] 郝凯凯 

机构地区：[1]延安大学生命科学学院,延安716000

出　　处：《安徽农业大学学报》2014年第5期875-881,共7页Journal of Anhui Agricultural University

基　　金：陕西省科技厅农业攻关项目(2012.k02-10);国家级大学生创新训练计划项目(201210719022);陕西省高水平大学建设专项资金生态学(2012SXTS03)共同资助

摘　　要：为了优化沙芥叶片总黄酮的提取工艺条件,以乙醇浓度、料液比、回流温度、回流时间为自变量,以总黄酮提取含量为响应值,应用Box-Behnken中心组合原理建立数学模型,进行响应面分析(RSM),确定总黄酮的最佳提取工艺。结果表明,沙芥叶片总黄酮的最佳提取工艺为:乙醇浓度85%,料液比1:28,提取温度85℃,回流时间2 h。经验证,沙芥总黄酮提含量实测值26.367 mg·g-1,与理论值的RSD为0.47%。说明采用响应面法得到的工艺参数可靠,具有一定的实际价值。To optimize the extraction process of total flavonoids from Pugionium cornutum leaves, the effectsof ethanol concentration, solid-liquid ratio, reflux temperature, and reflux time on the extraction yield of total fla-vonoids from leaves were determined using Response Surface Methodology （RSM）. Results showed that the op-timum technological conditions were： 85% ethanol concentration, 1：28 material-liquid ratio, 85℃ reflux tem-perature, and 2 h reflux time. Under such conditions, the actual value of total flavonoids extracted was 26.367mg.g-1 that was 0.47% of the theoretical value of RSD. The results suggested that the optimum process conditionsgenerated from RSM are reliable and practical.

关 键 词：沙芥 总黄酮 回流提取 响应面法 

分 类 号：R282[医药卫生—中药学]