黄药子及配伍甘草对大鼠肝脏CYP450基因表达的影响  

Effects of the compatibility of Huangyaozi and Gancao on the CYP450 mRNA expression in rat liver

作　　者：华碧春[1] 黄智锋[1] 刘娇[1] 程心玲[2] 陈小峰[3] 王英豪[1] 卓实[2] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建省人民医院,福建福州350004 [3]福建省第二人民医院,福建福州350003

出　　处：《中药与临床》2014年第4期12-14,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(No:81274105)

摘　　要：目的:考察甘草对黄药子引起的肝损伤的CYP450酶基因表达的影响。方法:采用逆转录聚合酶链反应(RTPCR)技术检测大鼠肝脏CYP1A2、CYP2C19的mRNA的表达。结果:在mRNA水平上,28天、35天黄药子组均显著诱导CYP1A2的基因表达,配伍甘草后表达降低,具有统计学意义。甘草组可微弱下调CYP2C19的表达,但与配伍组相比无统计学意义。结论:甘草通过抑制CYP1A2的mRNA表达,对黄药子致肝损伤具有一定保护作用。Objective： To study the effects of the compatibility of Huangyaozi and Gancao on the CYP450 mRNA expression in rat livers. Method： The mRNA levers of the expression of the CYP 1A2 and CYP2C 19 gene of the rat liver were assayed with RT-PCR. Result： The mRNA levels of CYP1A2 were induced significantly by group of Huangyaozi after medication for 28 days and 35 days separately and reduced after given the compatibility with Gancao. Conclusion： The mRNA expression of CYP1A2 was inhibited by Gancao to protect liver from injury which caused by Huangyaozi.

关 键 词：黄药子 甘草 CYP450 基因表达 配伍减毒 

分 类 号：R285.5[医药卫生—中药学]