高效液相色谱检测新型“香料”毒品中的两种合成大麻素  

Study on Determination of 2 Synthetic Cannabinoids in Novel “Spice”Drugs by High Performance Liquid Chromatography

作　　者：翟晚枫[1] 张春水[1] 高利生[1] 

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《中国药物滥用防治杂志》2014年第5期270-273,共4页Chinese Journal of Drug Abuse Prevention and Treatment

基　　金：公安部重点研究计划项目(201201ZDYJ005)

摘　　要：目的：建立了新型“香料”毒品中2种合成大麻素（ CP47，497和其同系物 CP47，497（C8））的液相色谱分析方法。方法：以甲醇作为提取溶剂。采用岛津 ODS-SP 型（250mm×4.6mm，5μm）色谱柱，以215nm 作为检测波长。以（甲醇-乙腈）-高纯水为流动相进行梯度洗脱，流速1.2mL/ min，柱温35℃，进样量10μL。结果：在优化条件下，CP47，497和 CP47，497（C8）在1-100μg/ mL 范围内线性良好，相关系数均大于0.9999。方法的检出限（S/ N≥3.3）为0.37μg/ mL 和0.49μg/ mL。日内精密度（相对标准偏差（RSD））为0.82%-1.32%，日间精密度为1.46%-2.11%。CP47，497和 CP47，497（C8）样品溶液在室温下60h 内稳定性良好。结论：方法快速、灵敏、准确、可靠，适用于新型“香料”毒品样本中 CP47，497和 CP47，497（C8）的定量测定。Objective：A high performance liquid chromatographic method was established for determination of CP47,497 and its homologue CP47,497（C8）in novel“spice”drugs. Methods：Methanol was used as the extracting solvent. The separation was per-formed on a Shimadzu ODS-SP column（250mm×4. 6mm,5μm）with gradient elution,using（methanol-acetonitrile）-water as mobile phase. Flow rate was set at 1. 2mL/ min,column temperature was 35℃ and injection volume was 10μL. The detection wavelength of UV detector was set at 215nm. Results：Good linearity was achieved over the range of 1 - 100μg/ mL,with correlation coefficients more than 0. 9999. Limit of detection（S/ N≥3. 3）was 0. 37μg/ mL and 0. 49μg/ mL. The intra-day relative standard deviations（RS-Ds）were 0. 82% - 1. 32% ,and the inter-day RSDs were 1. 46% - 2. 11% . The sample showed stability under room temperature in 60 hours. Conclusion：This method was rapid,sensitive and accurate,and was applicable to quantitative determination of CP47,497 and CP47,497（C8）in novel“Spice”drugs.

关 键 词：液相色谱 合成大麻素 CP47 497 

分 类 号：O657.72[理学—分析化学] TS102.224[理学—化学]