黄芩苷联合NB-UVB对黑素细胞黑素合成及酪氨酸酶活性的影响  

Influence of baicalin combined with NB-UVB on melanin synthesis and tyrosinase activity in melanocytes

作　　者：姚蕾[1] 钟淑霞[1] 兰珊珊[1] 宋洋[1] 周俊峰[1] 李珊山[1] 

机构地区：[1]吉林大学第一医院皮肤科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2014年第5期1024-1027,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(20110478);吉林省中医药管理局中医药科技项目资助课题(2006)

摘　　要：目的:观察体外培养的人正常黑素细胞经黄芩苷和窄波紫外线(NB-UVB)处理后细胞黑素合成及酪氨酸酶活性的变化,为白癜风的新疗法研究提供理论依据。方法:人正常黑素细胞分为空白对照组、不同浓度黄芩苷组、不同剂量NB-UVB组及黄芩苷和NB-UVB联合组。应用MTT法检测各组黑素细胞增殖率,应用NaOH裂解法测定黑素水平,应用多巴氧化法测定酪氨酸酶活性。结果:与空白对照组比较,1×10-4 mol·L-1黄芩苷及30mJ·cm-2 NB-UVB组黑素细胞增殖率差异无统计学意义(P>0.05)。与空白对照组比较,黄芩苷组、NB-UVB组及联合组黑素水平及酪氨酸酶活性均增加(P<0.05或P<0.01);与黄芩苷组和NB-UVB组比较,联合组黑素水平和酪氨酸酶活性均增加(P<0.01)。结论:黄芩苷与NB-UVB联合应用较2种方法单独应用具有更好的促黑素合成效果。Objective To observe the synthesis of melanin and tyrosinase activity of the cultured normal human melanocytes after treated with baicalin and NB-UVB, and to provide theoretical evidence for study on the new therapy method of vitiligo.Methods The normal human menlanocytes cultured in vitro were divided into control group,different concentrations of baicalin groups,different doses of NB-UVB group and baicalin combined NB-UVB group.The proliferation rate of melanocytes was measured by MTT assay,the level of melanin was tested by NaOH pyrolysis,and the tyrosinase activity was measured by dopa oxidization.Results Compared with control group,the proliferation rates in 10-4 mol·L-1 baicalin group and 30 mJ·cm-2 NB-UVB group had no significant difference（P〉0.05 ）. Compared with control group, the levels of melanin and tyrosinase activities in baicalin group,NB-UVB group and combined group were increased （P〈0.05 or P〈0.01）;compared with baicalin group and NB-UVB group,the level of melanin and tyrosinase activity in combined group were increased（P〈0.01）. Conclusion Baicalin combined with NB-UVB has better promotion effect on melanin synthesis than used alone.

关 键 词：黑素细胞 黄芩苷 窄波紫外线 黑素 酪氨酸酶 

分 类 号：R285[医药卫生—中药学] R75[医药卫生—中医学]