泻白散对小鼠过敏性哮喘气道炎症的作用及机制  

Effects and Its Mechanism of Xiebai San on Allergic Airway Inflammation in Asthma Mouse

作　　者：张天柱[1] 张景龙[1] 樊湘泽 程潜[1] 

机构地区：[1]长春中医药大学,长春130117

出　　处：《中国实验方剂学杂志》2014年第20期173-177,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省中医药科技项目(2012-083)

摘　　要：目的：评价泻白散对哮喘小鼠变应性气道炎症的影响.方法：BALB/c小鼠60只,随机分为正常组、模型组、泻白散高剂量组（12 g·kg^-1）、中剂量组（6 g·kg^-1）、低剂量组（3 g·kg^-1）、地塞米松阳性对照组（2 mg·kg^-1）,共6组.各组小鼠（除正常组外）于第1,8天腹腔、皮下分别给予0.1 mL的致敏液（0.2 mL致敏液含卵蛋白（OVA）0.1 mg,氢氧化铝0.02 mg）,第15 ～ 28天各组（除正常组）给予5％的OVA雾化激发,每次雾化激发20 min,每次雾化前30 min各组小鼠按体重给予相应的药物;空白组以等体积生理盐水代替致敏液,并用生理盐水雾化激发相同的时间.相应的药物治疗2周后,取血做嗜酸性细胞（EOS）计数,取肺泡灌洗液（BALF）做细胞分类计数.采用酶联免疫法（ELISA）法测定BALF中的白介素-6（IL-6）及肿瘤坏死因子α（TNF-α）含量.采用Western blot法检测肺组织GATA结合蛋白3（GATA3）与Th1细胞特异性转录因子（T-bet）蛋白表达.结果：泻白散高、中、低剂量组使哮喘小鼠血中EOS的含量明显降低（P＜0.01,P＜0.05）,对淋巴细胞及中性粒细胞没有明显的影响;使肺泡灌洗液中的EOS明显下降（P＜0.01,P＜0.05）,对肺泡灌洗液的单核细胞及中性粒细胞没有明显的影响;病理显示,各给药组小鼠肺部病变减轻.泻白散高、中、低剂量组能显著降低哮喘小鼠BALF中IL-6及TNF-α的含量,能显著降低哮喘小鼠肺部GATA3蛋白表达,并能提高哮喘小鼠肺部T-bet蛋白表达.结论：泻白散能有效的抗哮喘,其作用机制可能是通过调节GATA3与T-bet蛋白的表达.Objective：To investigate the effects and its mechanismof Xiebai San on allergic asthma.Method：Sixty BALB/c were divide into six groups：normal group,model groups,Xiebai San high dose （12 g ·kg^-1）,middle dose （6 g ·kg^-1）,low dose （3 g ·kg^-1）,dexamethasone positive group [（2 mg ·kg^-1）,groups mice （except those in the normal group） were sensitized with 0.2 mL sensitized solution （0.2 mL sensitized solution contains 0.1 mg OVA and 0.02 mg AI （OH）3）].Fifteen day after modeling,and corresponding drugs were given,30 min later,mice were stimulated with 5% OVA.Blood simples were collectesd for counting numbers of eosinophilic granulocyte （EOS）,and bronchoalveolar laverge fluid （BALF） was used for counting inflammatory cells.ELISA was used to assay interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） levels,Western blotting was Use to detect GATA3 and T-bet.Result：The middle dose of Xiebai San could obviously reduce numbers of EOS in the blood （P 〈 0.01）,high dose and low dose of Xiebai San could also reduce numbers of EOS （P 〈 0.05）,high and middle dose of Xiebai San could obviously reduce numbers of EOS in the BALF （P 〈 0.01）,low dose Xiebai San could also reduce numbers of EOS （P 〈 0.05）,and Xiebai San obviously reduced IL-6 and TNF-α,and GATA3,and increased T-bet.Conclusion：Xiebai San can inhibit allergic aasthma.

关 键 词：泻白散 过敏性哮喘 GATA3结合蛋白3 Th1细胞特异性转录因子 

分 类 号：R285.5[医药卫生—中药学]