玉米大斑病感、抗近等基因系SNP基因芯片分析  

SNP Gene Chip Analysis of Near-isogenic Lines to North Corn Leaf Blight

作　　者：马骏[1] 王延波[1] 刘欣芳[1] 李明[1] 弓雪[1] 齐欣[1] 姜敏[1] 

机构地区：[1]辽宁省农业科学院玉米研究所,沈阳110161

出　　处：《玉米科学》2014年第5期153-158,共6页Journal of Maize Sciences

基　　金：国家自然基金面上项目(31371627);辽宁省自然科学基金项目(2013010337-401);农业部转基因生物新品种培育重大专项(2013ZX08003004-007)

摘　　要：通过SNP基因芯片(56 110个SNP)对A619感、A619Ht3抗玉米大斑病近等基因系进行分析,采用生物信息学和比较基因组学方法进行显著SNP位点候选基因筛查和功能预测。结果表明,发现10个热点区域,分别位于bin2.06、3.02/3.03、3.04、3.07、3.08、7.03和bin9.08,38个SNP被确定与大斑病抗性相关;挖掘出24个候选基因,通过注释发现,候选基因多与各种受体类酶活性、金属离子及能量的代谢和平衡等功能相关。A pair near-isogenic lines of north corn leaf blight using of SNP gene chip（56 110 SNP） was ana- lyzed. Through bioinformatics and comparative genomics analysis, potential genes screening and function prediction were performed. Thirty-nine SNP were identified associated with resistance, ten candidate area were positioned, lo- cated bin2.06, 3.02/3.03, 3.04, 3.07, 3.08, 7.03 and bin9.08. Twenty-four candidate genes were digged out. The function included a variety of kinase activity, metal ion,energy metabolism and balance.

关 键 词：玉米 玉米大斑病 抗性基因 SNP 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]