玉葵清对糖基化终产物诱导的人肾系膜细胞趋化因子表达的影响  

Effect of YuKuiQing on the expression of monocyte chemoattractant protein-1 and fractalkine induced by AGEs

作　　者：孙红[1,2] 袁扬[1,2] 周莉[3] 余江毅[4] 孙子林[1,2] 

机构地区：[1]东南大学附属中大医院内分泌科 [2]东南大学医学院糖尿病研究所,南京210009 [3]淮安市第一人民医院 [4]江苏省中医院内分泌科

出　　处：《中国糖尿病杂志》2014年第10期942-946,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金青年项目(81200635);江苏省自然科学基金面上项目(SBK201122155)

摘　　要：目的探讨玉葵清对糖基化终产物(AGEs)诱导的人肾系膜细胞(HRMC)趋化因子表达及其趋化效应的影响。方法糖基化牛血清白蛋白(AGE-BSA)和玉葵清干预HRMC。结果 AGEBSA组较BSA组HRMC趋化单核细胞数增加,单核细胞趋化蛋白-1(MCP-1)、Fractalkine(FKN)中和抗体组HRMC趋化单核细胞数较AGE-BSA组减少;玉葵清组MCP-1、Fractalkine基因表达、上清中的蛋白含量和趋化单核细胞较BSA组降低(P<0.05)。结论玉葵清减弱AGE-BSA诱导的HRMC趋化因子表达及其趋化效应,可能改善DN肾脏炎症反应。Objective To further investigate the effect of YuKuiQing on the upregulation of monocyte chemoattractant protein-1 （MCP-1） and fractalkine （FKN） induced by AGEs.Methods Human renal mesangial cells （HRMC） was cultured in the presence of AGE-BSA with or without antiMCP-1 and anti-FKN antibody or YuKuiQing.The capacity of MCP-1 and FKN to induce monocytes transmigration was detected with a transwell system.The MCP-1 and FKN mRNA and protain in HRMC were analyzed by RT-PCR and ELISA.Results （1）The number of monocytes migrating to HRMC was higher in AGE-BSA group than in control and anti-MCP-1 or anti-FKN antibody-blocking groups（P＜ 0.01）.（2） The number of monocytes migrating to HRMC was lower of YuKuiQing than in control and AGE-BSA （P＜0.01）group.（3）Expression of MCP-1 and FKN mRNA significantly lower in YuKuiQing group than in control group （P＜0.01） and AGE-BSA（P＜0.05） group.（4） In YuKuiQing group,the contents of MCP-1 and FKN in supernatant were significantly decreased compared to control and AGE-BSA groups （P＜0.01）.Conclusion AGE-BSA enhances the capacity of MCP-1 and FKN to induce monocytes transmigration to HRMC,which are inhibited by anti-MCP-1 and anti-FKN antibody partially.These findings suggest that MCP-1 and FKN might mediate the role of AGEs in the development of diabetic nephropathy.YuKuiQing can markedly reduce the upregulation of MCP-1 and FKN induced by AGE-BSA,and attenuate the capacity of MCP-1 and FKN to induce monocytes transmigration to HRMC.These results indicate that YuKuiQing might play its role of renal protection.

关 键 词：糖基化终产物 单核细胞趋化蛋白-1 FRACTALKINE 玉葵清 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌]