柱前衍生HPLC法分析蔓菁多糖中单糖的组成  

Analysis of monosaccharide compositions in Brassica rapa polysaccharide by precolumn derivation HPLC

作　　者：邝婷婷[1] 王宇[1] 王张[1] 杨永东[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137

出　　处：《中成药》2014年第10期2121-2125,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81403187);四川省中医药管理局科技专项(2012-G-029);成都中医药大学科技发展基金(CGZH201201);四川省教育厅四川省省属高校科研创新团队建设计划(11TD004)

摘　　要：目的建立柱前衍生HPLC法测定藏药蔓菁多糖中单糖的组成。方法以2 mol/L硫酸水解蔓菁多糖,水解产物加入1-苯基-3-甲基-5-吡唑啉酮进行衍生化,采用反相高效液相色谱法,测定蔓菁多糖中单糖的衍生物。Kromasil C18色谱柱(250 mm×4.6 mm,5μm);乙腈为A流动相,0.1 mol/L磷酸盐(KH2PO4-Na OH,p H 6.8)缓冲液为B流动相,梯度洗脱;检测波长250 nm。结果蔓菁多糖由半乳糖、D-甘露糖、鼠李糖、阿拉伯糖、D-无水葡萄糖、D-葡萄糖醛酸、D-半乳糖醛酸7种单糖组成,其摩尔比为1∶0.26∶0.54∶1.06∶0.84∶0.05∶4.17。结论柱前衍生HPLC法表明蔓菁多糖主要由半乳糖醛酸、半乳糖和阿拉伯糖构成。AIM To establish a pre-column derivation HPLC method for determining monosaccharide composi- tions in Brassica rapa polysaccharide. METHODS The polysaccharide was hydrolyzed with 2 mol/L sulfuric acid and derived by 1-phenyl-3-methyl-5-pyrazolone （PMP）. The analysis of monosaccharide composition of Brasscia rapa polysaccharide was carried out by reversed-phase HPLC on a Kromasil Cls column with a mobile phase composed of 0. 1 mol/L phosphate （KH2PO4-NaOH, pH 6. 8） and acetonitrile in a gradient elution manner. The detection wave- length was set at 250 nm. RESULTS The Brassica rapa polysaccharide was composed of galactose, D-mannose, rhamnose, arabinose, D-glucose, D-glucuronic acid and D-galacturonic acid with a molar ratio of 1 ： 0. 26 ： 0. 54 ： 1.06 ： 0. 84 ： 0. 05 ： 4. 17. CONCLUSION The HPLC method with pre-column derivatization shows that Brasscia rapa polysaccharide mainly consists of galacturonic acid, galactose and arabinose.

关 键 词：藏药 蔓菁多糖 柱前衍生化 单糖 

分 类 号：R284.1[医药卫生—中药学]