rhGM-CSF和纳米银联合外用对深Ⅱ°烫伤创面炎性反应的影响  

Effect of recombinant human granulocyte/macrophage colonystimulating factor combined with nano-silver on deep burn degreen Ⅱ about inflammation

作　　者：杨景哲[1] 耿琪瑛[1] 冯欣姝[1] 陈凤平[1] 王芳[1] 

机构地区：[1]承德医学院附属医院烧伤整形科,河北承德067000

出　　处：《重庆医学》2014年第29期3926-3928,共3页Chongqing medicine

基　　金：承德市科学技术研究与发展计划资助项目(20122164);河北省2013年医学科学研究重点课题计划指令性课题(20130024)

摘　　要：目的研究外用重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和纳米银敷料联合外用对深Ⅱ°烫伤创面愈合过程炎性反应的影响。方法用Wistar大鼠建立深Ⅱ°烫伤模型,分为A、B、C、D 4组,A组30例:凡士林纱布覆盖;B组30例:纳米银敷料覆盖;C组30例:rhGM-CSF涂抹创面;D组30例:rhGM-CSF联合纳米银敷料覆盖,伤后第1、4、7、10、14、21天,观察创面炎性反应,细菌培养并计数,按照ELISA测定血清IL-2、IL-8水平。结果炎性反应由重至轻依次为A组、B组、C组、D组;A组、B组/C组、D组分别于伤后第4、10、14天出现细菌生长,D组与A、B、C组比较,B、C组与A组比较,细菌生长数量少,且第10、14、21天差异有统计学意义(P<0.05);各组间IL-2、IL-8水平各时相点不同,D组最低,A组最高;IL-2水平,除伤后第1天各组间差异无统计学意义,第4天,B组与C组间差异无统计学(P>0.05)外,其余各时相点各组间差异有统计学意义(P<0.05);IL-8水平,除第1天,A组与B组、B组与C组、D组与C组间差异无统计学意义(P>0.05)外,在各时相点各组间差异均有统计学意义(P<0.05)。结论 rhGM-CSF和纳米银联合外用,减轻深Ⅱ°烫伤创面炎性反应,并且优于rhGM-CSF、纳米银单独应用。Objective To observe the effect of recombinant human granulocyte/macrophage colonystimulating factor （rhGM-CSF） combined with nano-silver as a treatment on deep burn degreen Ⅱ about inflammation .Methods The burn model was built with Wistar rats .They were randomly divided into four groups ,petrolatum treatment （group A ,n= 30） ,nano-silver treatment （group B ,n=30） ,rhGM-CSF treatment（group C ,n=30） ,and rhGM-CSF combined with nano-silver treatment（group D ,n=30） . observation the inflammatory reaction ,and culture bacteria on wound of the four groups at 1st ,4th ,7th ,10th ,14th ,21th day after treatment were made .The level of IL-2 and IL-8 were measured in serums with ELISA .Results The inflammatory reaction：group A〉group B〉group C〉group D ;Bacterias were observed in group A ,group B/C and group D at 4th ,10th ,14th day respectively af-ter treatment .The number of bacterial growed in group D was less than in group A ,group B and group C .The numbers of bacterial growed in group B and group C were less than in group A .And after 10 ,14 ,21 days treatment ,there was significantly statistical difference（P〈0 .05） .There was difference among groups in the levele of IL-2 and IL-8 ,which were the lowest in group D and the highest in group A .The level of IL-2 has no significantly statistical difference between every groupat 1st day and between B and C group at 4th day（P〉0 .05） .After other days treatment ,there was significantly statistical difference（P〈0 .05） .There was signifi-cantly statistical difference in every group at each day of the IL-8 levele except A and B group ,B and C group ,C and D group at 1st day （P〈0 .05） .Conclusion rhGM-CSF combined with nano-silver treatment could alleviate inflammatory reaction ,and be better than rhGM-CSF or nano-silver alone .

关 键 词：DNA 重组 巨噬细胞集落落刺激因子 银 烧伤 

分 类 号：R644[医药卫生—外科学]