五味子甲素SchA缓解MPP+诱导的SH-SY5Y细胞损伤的机制研究  

Investigation of mechanism of SchA to decreasing MPP+-induced SH-SY5Y cell damage

作　　者：纪朋艳[1] 李妍[2] 张巍[2] 彭顺利[3] 何哲[4] 

机构地区：[1]吉林医药学院生物化学实验室,吉林吉林132013 [2]吉林医药学院生物化学教研室,吉林吉林132013 [3]吉林医药学院临床医学院,吉林吉林132013 [4]吉林医药学院药学院,吉林吉林132013

出　　处：《重庆医学》2014年第29期3932-3934,共3页Chongqing medicine

基　　金：吉林省科技厅科研项目(201205079);吉林市科技局科研项目(201162511)

摘　　要：目的探讨五味子甲素(SchA)缓解1-甲基-4-苯基吡啶离子(MPP+)诱导SH-SY5Y细胞损伤的可能机制。方法实验分为5组,空白对照组,加入无血清培养基进行培养;模型组(MPP+进行诱导);SchA组;SchA与MPP+共同处理的SchA组,其中SchA终浓度分别为1、3、5μmol/L,MPP+终浓度为1mmol/L。NO检测试剂盒检测各组细胞NO含量,Western blot检测细胞总Akt、p-Akt的蛋白表达情况。结果与对照组相比,1mmol/L MMP+诱导后,SH-SY5Y细胞NO含量明显增高(P<0.05),经5μmol/L SchA及MPP+共同处理的SH-SY5Y细胞,NO的含量明显降低。各组总Akt相对表达水平没有明显变化(P>0.05);模型组细胞p-Akt相对表达水平较对照组减少,SchA组细胞p-Akt蛋白相对表达水平随SchA浓度的升高而升高,且与模型组比较差异都有统计学意义(P<0.05)。结论 SchA缓解MPP+诱导的SH-SY5Y细胞损伤可能与影响NO含量及p-Akt蛋白表达有关。Objective To explore the possible mechanism of SchA ,which decreases MPP＋induce SH-SY5Y cell damage .Meth-ods Cultured cells were divided into 5 groups ,one as control group ,cultured by free-blood serum media;the other 4 groups were treated with different concentrations of SchA（1 ,3 ,5 μmol/L） and MPP＋ （1 mmol/L） for 48 h named model group ,1 ,3 ,5 μmol/L SchA group respetivly .The content of nitric oxide（NO） were measured by NO kit ;The expression levels of total Akt and p-Akt proteins were detected by Western blot .Results Compared with the control group ,the content of NO in group significantly in-creased after MPP＋stimulating（P〈0 .05）;compared to the control group ,the content of NO in 5μmol/L SchA group significantly decreased（P〈0 .05） .The expression levels of total Akt in all groups had no significant difference（P〉0 .05） .The expression levels of p-Akt in model group significantly lowered ,while SchA（1、3、5 μmol/L） significantly increased the expression levels of p-Akt in comparision with cells in model group .Conclusion Decreasing MPP＋ induced SH-SY5Y cell damage of SchA may be related to the content of NO and p-Akt expression .

关 键 词：五味子素 1-甲基-4-苯基吡啶离子 S H-SY5Y细胞 蛋白激酶类 

分 类 号：R285[医药卫生—中药学]