木榄细胞膜Na^+/H^+逆向运输蛋白BgSOS1功能的初步验证  

Preliminary Analysis of a Na^+/H^+ Antiporter BgSOS1 at Plasma Membrane of Bruguiera gymnorrhiza

作　　者：荆海瑜[1] 周扬[1] 郭晓颖[1] 从心黎[2] 黄绵佳[2] 江行玉[1] 

机构地区：[1]海南大学海南省热带作物资源可持续利用重点实验室,海南海口570228 [2]海南大学园艺园林学院,海南海口570228

出　　处：《海南大学学报（自然科学版）》2014年第3期252-259,269,共9页Natural Science Journal of Hainan University

基　　金：国家自然科学基金项目(31260218);海南省自然科学基金项目(313030);海南大学科研启动项目(Kyqd1205)

摘　　要：根据木榄BgSOS1的序列信息从木榄中克隆到了全长的BgSOS1基因,该片段包含1个3 462 bp的开放阅读框,可编码1 153个氨基酸的多肽.生物信息学的分析结果表明:该氨基酸序列所编码的蛋白与拟南芥、番茄、水稻、小麦、盐芥和杨树的SOS1蛋白高度同源,同源性分别为63.86%,66.61%,62.18%,60.19%,63.97%和75.97%;BgSOS1蛋白的N端含有12个跨膜的结构域,C端为1个较长的胞内结构域.尽管单个BgSOS1的表达并不能提高转基因酵母的抗盐性,但SOS1,SOS2和SOS3共表达的酵母,其抗盐性明显提高,这表明SOS2/SOS3蛋白激酶复合体可能通过调控BgSOS1的Na+/H+交换功能来提高木榄的抗盐机理.According to the sequence information from the GenBank,the full length BgSOS1 gene with 3462 bp was cloned,which encodes a protein of 538 amino acids. The analysis results of multiple alignments of the deduced amino acid sequences of BgSOS1 with the SOS1 in six other plants showed that sequence identities between them are 63. 86% with Arabidopsis SOS1,66. 61% with tomato SOS1,62. 18% with rice SOS1,60. 19% with wheat SOS1,63. 97% with salt cress SOS1 and 75. 97% with poplar SOS1,respectively. It were predicated that there are twelve putative transmembrane domains in N end of the protein and there is a long cytoplasm domain in C end. The expression of BgSOS1 gene did not increase the tolerance of yeast strain AXT3 K to salt stress,however,the salt tolerance of AXT3 K co-transformed with BgSOS1,AtSOS2 and AtSOS3 was significantly higher than that of non-transgenic yeast strain,which suggested that Na^＋/H^＋antiport activity of SOS1 might be regulated through protein phosphorylation by SOS2 /SOS3 kinase complex.

关 键 词：Na^+/H^+逆向运输蛋白 细胞膜 木榄 抗盐机理 

分 类 号：S311[农业科学—作物栽培与耕作技术]