黄芪六一汤对糖尿病大鼠血糖、胰岛素、糖化血红蛋白、细胞形态影响随机平行对照研究  

Randomized Parallel Group Study on the Effect of Huangqiliuyi Decoction on Blood Sugar,Insulin,Glycated Hemoglobin,Cell Morphology of Diabetic Rats

作　　者：陈玉兴[1] 黄雪君[1] 杜铁良[1] 曾晓会[1] 姚楠[1] 黄丹娥[1] 

机构地区：[1]广东省中医研究所,广州510095

出　　处：《实用中医内科杂志》2014年第9期113-116,134,共5页Journal of Practical Traditional Chinese Internal Medicine

摘　　要：[目的]观察黄芪六一汤对糖尿病大鼠血糖及胰腺影响。[方法]采取使用随机平行对照方法,将90只SPF级SD大鼠,按随机数字表方法随机分为9组(10只/组);80只大鼠按李大伟方法复制STZ诱导糖尿病大鼠模型,选取血糖居中合格大鼠60只,按随机数字表法随机分6组,模型组、二甲双胍组、消渴丸组、黄芪六一汤(高、中、低剂量)组;造模后第7d分别灌胃干预,黄芪六一汤(生药12.60g/kg,6.30g/kg,3.15g/kg),二甲双胍0.17g/kg,消渴丸0.63g/kg;正常组、模型组同法灌胃等体积生理盐水。连续灌胃干预给药4周后,末次给药前各组大鼠禁食16h,末次给药1h后,常规眼眶静脉丛取血;采血后大鼠脱颈椎处死,取胰腺作病理检查。观测血糖、胰岛素、糖化血红蛋白、细胞形态。[结果]血糖各造模组均高于空白组(P<0.01),血糖值≥13mmolL-1;黄芪六一汤(高、中、低剂量)组血糖均明显低于模型组(P<0.01)。糖化血红蛋白模型组高于空白组(P<0.01),胰岛素含量模型组低于模型组(P<0.01);糖化血红蛋白黄芪六一汤(高、中、低剂量)组明显低于模型组(P<0.01),胰岛素高于模型组(P<0.05)。病理检查空白组胰岛多为圆形或椭圆形细胞团,分散于胰腺腺泡之间,胰岛边界清晰,无包膜,细胞团大小不一,胰岛细胞为多边形,排列整齐,分布均匀,胞浆淡染呈浅粉红色,核多为圆形深染;模型组胰岛细胞可见不同程度肿胀和空泡样变性,边界不清,细胞核大小不等,细胞数量明显减少,排列紊乱,部分细胞发生核固缩和坏死;二甲双胍、消渴丸组胰岛细胞形态结构明显改善,细胞数目增多,排列较整齐,胞浆分布均匀,部分细胞发生肿胀和空泡变性;黄芪六一汤(高、中、低量)组胰岛细胞形态结构明显改善,胰岛细胞肿胀和空泡样变性的程度明显减轻,细胞数目增多,排列较整齐。[结论]黄芪六一汤能显著降低糖尿病大鼠空腹血糖、糖化血红蛋白,升高�[ Objective ] To observe the effects of blood glucose and pancreas of Huangqiliuyi Decoction on experimental diabetic rats. [ Methods ] Taking random parallel control method, 90 SPF SD rats were randomly divided into 9 groups （ 10 / group ） ; 80 rats were Dawei way to copy STZ-induced diabetic rat model, select glucose centering pass 60 rats were randomly divided into 6 groups, model group, metformin group, Xiao Ke Wan group, Huangqiliuyi Decoction group （ high, medium and low dose ） group;Rats were first garage drugs after Modeling 7d, Huangqiliuyi Decoction （crude drug 12.60g/kg, 6.30g/kg, 3.15g/kg）, mefformin O.17g/ kg, Xiao Ke Wan 0.63g/kg;Normal group, model group with an equal volume of saline gavage method, after Continuous intragastric administration of four weeks , the rats fasted for 16h before the last administration , after the last administration lh, Blood was taken from the orbital venous plexus;Rats were sacrificed by cervical dislocation after blood collection, Pancreas removed for histological examination. Observation of blood glucose, insulin, glycated hemoglobin, cell morphology. [ Result ] The blood glucose of each model group were higher than control group （P〈0.01）, blood glucose≥13mmol·L^-1. The blood glucose of Huangqiliuyi decoction group （ high, medium and low dose ） were significantly lower than the model group （P 〈0.01 ） ;The glycated hemoglobin of model group were higher than the control group （ P 〈0.01 ）, the insulin levels of the model group were lower than the control group （P 〈 0.01 ）;The glycated hemoglobin of Huangqiliuyi decoction group （ high, medium and low dose ） was significantly lower than that in the model group （ P 〈0.01 ）, insulin higher than the model group （ P〈0.05 ） . Pathological examination the control group islets were round or oval cell clusters, distributed between the pancreatic acinar, islet clear boundaries, no envelope, islet ceils are polygonal, arranged in neat, evenly distributed, cytoplasmic were

关 键 词：糖尿病大鼠 动物模型 黄芪六一汤 太平惠民和剂局方 血糖 胰岛素 糖化血红蛋白 细胞形态 实验研究 随机平行对照研究 

分 类 号：R255.4[医药卫生—中医内科学] R285.5[医药卫生—中医学]