氘代同位素稀释液相色谱-质谱法快速检测动物组织中11种β_2兴奋剂  

Rapid determination and confirmation of 11 β_2 - agonists in animal tissues by deuterium isotope dilution liquid chromatography- mass spectrometry

作　　者：贾玉珠[1] 白艳艳[1] 陈丽惠[1] 贾沁一[2] 

机构地区：[1]厦门市疾病预防控制中心,福建厦门361021 [2]北京化工大学,北京100029

出　　处：《中国卫生检验杂志》2014年第18期2585-2588,共4页Chinese Journal of Health Laboratory Technology

基　　金：厦门市科技计划指导性项目资助(3502z20089027)

摘　　要：目的建立LC/MS/MS法同时检测动物组织中11种β2兴奋剂的方法。方法食品样品添加内标溶液后经酶水解、乙酸钠溶液提取,MCX阳离子交换柱净化,在MRM模式下用LC/MS/MS测定动物组织中11种β2兴奋剂的含量。结果 11种β2兴奋剂在猪肉中的加标回收率为80%~107%,相对标准偏差为5.5%~11.8%;在0.5μg/L^200μg/L浓度范围内相关系数>0.9961;方法检出限<0.05μg/kg、定量限<0.2μg/kg。结论该法具有简单、快速、灵敏等优点,并可同时对动物性食品中多种β2受体激动剂进行定性确证和定量测定,回收率达到较好水平,适合对动物性食品中β2受体激动剂残留的分析检测。Objective To establish a method for simultaneous detection of 11 β2- agonists in animal tissues by LC- MS /MS.Methods The animal tissues were enzymatic hydrolyzed,extracted with sodium acetate solution and finally purified with a MCX cation- exchange column. The quantification was determination by LC- MS /MS in multiple reaction monitoring（ MRM）mode using isotope dilution internal standard method. Results The recoveries for 11 β2- agonists in pork samples were 80%to 107%,and the relative standard deviations（ RSDs） ranged was 5. 5% to 11. 8%. The linearities of the method were in the range of 0. 5 μg /L ~ 200 μg /L,with the correlation coefficients（ r） of greater than 0. 9961. The limits of detection were less than 0. 05 μg /kg and the limits of quantification were less than 0. 2 μg /kg. Conclusion This method is simple,rapid,and sensitive. It achieves the detection and confirmation of various β2- agonists in animal tissues simultaneously with a respectively good recovery level. Therefore,this method is suitable for determination of β2- agonist residues in animal tissues.

关 键 词：三重四级杆液质联用仪 动物组织 β2-兴奋剂 氘代同位素 

分 类 号：O657.63[理学—分析化学]