基于Cu^(2+)和碳纳米管纳米复合物的过氧化氢生物传感器测定过氧化氢  

Determination of hydrogen peroxide with hydrogen peroxide biosensor based on Cu^(2+)/carbon nanotubes composite

作　　者：白祖海[1] 张瑾 吾尔叶提 

机构地区：[1]长沙市疾病预防控制中心,湖南长沙410000 [2]吐鲁番疾病预防控制中心,新疆吐鲁番838000

出　　处：《中国卫生检验杂志》2014年第18期2621-2624,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的将Cu2+和碳纳米管(MWNTs)固定于脱氧核糖核酸(DNA)和聚丙烯胺(PAA)中,形成MWNTs/Cu2+-DNA/PAA纳米复合物,并用此纳米复合物构建了一种新的过氧化氢生物传感器。方法将该纳米复合物修饰玻碳电极应用于过氧化氢的测定。结果 Cu2+和MWNTs之间对过氧化氢的还原具有协同电催化作用。在pH=5.0的磷酸盐缓冲溶液中,与DNA/PAA复合物中只存在Cu2+或MWNTs的复合物修饰玻碳电极相比,对过氧化氢的还原电催化稳态电流分别提高了约7倍和2.5倍。所制备的生物传感器对过氧化氢的线性范围为5.0μmol/L^4 mmol/L,检测下限为2.5μmol/L(S/N=3)。结论在电极表面涂覆一层Nafion膜可以有效消除抗坏血酸、尿酸对过氧化氢的测定影响。这种新型传感器的优越性在于它可以克服天然酶易失活、不稳定的缺点而具有良好的重现性与稳定性。Objective New amperometric hydrogen peroxide biosensor was fabricated by integrating the Cu2 ＋and multi- wall carbon nanotubes（ MWNTs） in a DNA and poly（ allylamine）（ PAA） complex to form new MWNTs /Cu2 ＋- DNA /PAA nanocomposites. Methods The hydrogen peroxide biosensor based on Cu2 ＋/carbon nanotubes composite was used to determinate hydrogen peroxide. Results The presence of Cu2 ＋and MWNTs showed synergistic electrocatalytic effect toward deoxidizing of hydrogen peroxide. At an applied potential of- 0. 2 V versus Ag /AgCl in air- saturated PBS solution（ pH 5. 0）,the current response of hydrogen peroxide at the DNA- Cu2 ＋/PAA and MWNTs /DNA /PAA composite modified GCE was increased by about 7 and 2. 5 times,respectively. The linear ranges of the well developed biosensor were 5. 0 μmol /L ~ 4 mmol /L with a detection limit of 2. 5 μmol /L estimated at a signal- to- noise ratio of 3. Conclusion The interferences of ascorbic acid and uric acid could be effectively inhibited by further modification of an outer layer of Nafion film. Furthermore,such sensor could overcome the disadvantages of native enzyme＇s inactivation and instability and exhibit good reproducibility and stability.

关 键 词：过氧化氢 协同电催化作用 电流型生物传感器 纳米复合物 

分 类 号：O614.121[理学—无机化学]