2种方法对淋巴细胞转化试验结果的影响  

Effect on experimental results of lymphocyte transformation by two methods

作　　者：郝燕[1] 

机构地区：[1]山西医科大学汾阳学院病原生物与免疫学教研室,山西汾阳032200

出　　处：《中国卫生检验杂志》2014年第18期2681-2682,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的淋巴细胞转化试验是免疫学实验常开项目之一,也是医学研究中常用的方法,而不同方法处理往往影响试验的结果。本文采用2种常用的试验方法来处理标本,比较其对试验结果的影响。方法使用淋巴细胞培养液培养外周血后,将标本分别用不同的方法处理,一种是洗涤细胞后直接推片,一种是固定液处理后滴片,将片子进行瑞氏染色后采用形态计数法检测淋巴细胞的转化率。结果 2种方法有明显的差异,第2种方法转化率偏高,说明不同方法对试验结果有影响。结论作为实验室教学采用第一种方法较好,省时省力。Objective Lymphocyte transformation test is a common immunology experiment and commonly used in medical research. Different treatment methods may influence the outcome of the test. Therefore,two commonly- used test methods were selected to deal with the samples to compare their individual effects on test results. Methods The peripheral blood samples were cultured in lymphocyte culture liquid,then treated with different methods respectively. One was directly pushing the cell sediments by a smear after cell suspension centrifugation,while the other was dropping the cell sediment solution on a smear after adding stationary liquid,then following Wright staining,the lymphocyte transformation rates were detected by morphological counting method. Results The two methods showed obvious differences,and the lymphocyte transformation rate of the second method was higher than the first method. It was proved that different methods can influence the test results. Conclusion The first method is better for laboratory teaching,time- saving and labour- saving.

关 键 词：淋巴细胞 转化 形态 

分 类 号：R446.6[医药卫生—诊断学]