亚洲黑熊主要组织相容性复合体DQA及DRB基因外显子2多态性分析  

Polymorphic Analysis of MHC-DQA and MHC-DRB Gene Exon 2 in Asiatic Black Bear(Ursus thibetanus)

作　　者：高文杰[1,2] 朱自严[2] 

机构地区：[1]华东师范大学,上海200241 [2]上海市血液中心,上海200051

出　　处：《中国畜牧兽医》2014年第10期157-164,共8页China Animal Husbandry & Veterinary Medicine

基　　金：卫生行业科研专项(201002005)

摘　　要：试验旨在分析亚洲黑熊主要组织相容性复合体(major histocompatibility complex,MHC)DQA和DRB基因外显子2多态性。采用PCR扩增和克隆测序等方法对来自云南地区的40只亚洲黑熊DNA样本进行研究。结果发现,在40只亚洲黑熊样本中检测出了9个DQA外显子2等位基因和13个DRB外显子2等位基因,和大熊猫相比,亚洲黑熊在DQA和DRB基因外显子2位点上有较高的多态性。通过对推导出的氨基酸序列抗原结合位点的同义替换和非同义替换的比较,证明了DQA和DRB位点的平衡选择作用。In this study,we analyze the allelic diversity of the DQAand DRBgene exon 2locus on major histocompatibility complex（MHC）gene of Asiatic Black bear.Researced by cloning,sequencing and statistical analysis in the 40 Asiatic Black bear（Ursus thibetanus）from Yunnan province.The results showed that nine alleles of the DQAgene exon 2and thirteen alleles of the DRBgene exon 2were identified from 40 individuals.Relative to the giant panda,the diversity of DQA and DRB locus in Asian Black bears were more polymorphic.Comparisons of non-synonymous and synonymous substitutions in the antigen binding sites of deduced amino acid sequences indicated evidence for balancing selection on DRB and DQA locus.

关 键 词：亚洲黑熊 DQA基因 DRB基因 多态性 平衡选择 

分 类 号：S813.3[农业科学—畜牧学]