澳洲白绵羊美丽臀和肌肉生长抑制素基因基因型的检测  

Detection of CLPG and MSTN Genes Genotypes on AWF Sheep

作　　者：张淑珍[1] 张莉[1] 赵福平[1] 王慧华[1] 刘瑞凿 刘真[1] 樊红樱 魏彩虹[1] 杜立新[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2014年第10期172-178,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划项目(2011BAD28B05-2)

摘　　要：美丽臀(callipyge,CLPG)及肌肉生长抑制素(myostatin,MSTN)是全世界公认的与肌肉生长发育相关的基因,突变后分别产生"美丽臀"和"双肌"的优良性状。为研究国外进口品种澳洲白绵羊是否携带2种基因的突变位点,本试验以澳洲白绵羊为试验动物,采用PCR-RFLP技术对CLPG、MSTN基因的多态性进行分型。结果发现:CLPG基因上有1个C→T的SNP位点,澳洲白绵羊群体中有CC和CT 2种基因型;MSTN基因在3′-UTR区发现1个G→A的SNP位点,澳洲白绵羊群体中有GG和GA 2种基因型。用R语言程序对所测数据进行性别间不同基因型与生长发育状况的单因素方差分析得知,CLPG和MSTN基因不同基因型对澳洲白公羊体重、体高、体斜长、尻宽、背膘厚和眼肌面积均无显著性影响(P>0.05);CLPG基因CT基因型在母羊体重上显著高于CC基因型(P<0.05),在体斜长上极显著高于CC基因型(P<0.01),MSTN基因GA基因型母羊眼肌面积极显著高于GG基因型(P<0.01)。澳大利亚进口澳洲白绵羊中筛选到含有CLPG和MSTN基因的突变位点,且该突变位点对绵羊体尺性状有显著性作用,因此澳洲白绵羊引进后可以作为改良本地羊品种、基因聚合及三元配套系的终端父本。Callipyge（CLPG）and myostatin（MSTN）are recognized as the growth of muscle-related genes in the world,which produce＂beautiful buttocks＂and＂double-muscle＂good traits after mutation.To study whether imported AWF sheep carrying mutations in two genes,we used PCR-RFLP technique to check.The results showed that there was a C→T SNP in CLPGgene,AWF sheep populations had CC and CT genotypes;A G→A SNP locus in the 3′-UTR region of MSTNgene,AWF sheep populations had GG and GA genotypes.Analysis of variance with the R language between different genotypes and growth in different gender,MSTN and CLPG of different genotypes on the AWF rams weight,height,length,rump width,fat thickness and loin area were no significant differences（P〉0.05）;CLPG CT genotype on ewe weight was significantly higher than CC genotype（P〈0.05）,in length was very significantly higher than CC genotype（P〈0.01）,MSTN GA genotype on ewe loin area was very significantly higher than GG genotype（P〈0.01）.We detected contain CLPG and MSTN mutations in imported AWF sheep from Australia,and the mutation of sheep body traits had significant effects.Therefore,the AWF sheep could used as improved local sheep breeds,gene pyramiding and terminals supporting male in ternary system after the introduction.

关 键 词：澳洲白绵羊 美丽臀 肌肉生长抑制素 PCR-RFLP 

分 类 号：S813.3[农业科学—畜牧学]