脂肪酸合成酶基因mRNA在马身猪和大白猪3种组织中的发育性表达研究  

Study on Developmental Expression of FAS Gene mRNA in Three Tissues of Mashen Pig and Large White Pig

作　　者：马丽[1] 高鹏飞[1] 石建中[2] 牛姣艳 黎威[1] 张燕青[1] 徐凯[1] 王泽艺 刘宏[2] 郭晓红[1] 李步高[1] 曹果清[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]大同市种猪场,山西大同037000

出　　处：《中国畜牧兽医》2014年第10期198-204,共7页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技攻关项目(20140311020-5;20080311031);山西省留学人员科技活动择优资助项目(614193);山西省高等学校优秀青年学术带头人支持计划;山西农业大学"中青年学术带头人及学术骨干"支持计划;山西省研究生优秀创新项目(20143099)

摘　　要：为探究脂肪酸合成酶(fatty acid synthase,FAS)基因在猪不同组织中的发育性表达规律,本研究采用实时荧光定量PCR技术检测马身猪和大白猪7个阶段(初生、30、60、90、120、150和180日龄)肝脏、背最长肌和背部皮下脂肪3种组织中FAS基因mRNA的相对表达量。结果表明,品种间比较,FAS基因mRNA在马身猪和大白猪肝脏、背最长肌和背部皮下脂肪组织各生长发育阶段中的表达差异均达到显著或极显著(除肝脏组织初生阶段和背部皮下脂肪组织120日龄阶段)(P<0.05;P<0.01)。FAS基因mRNA在大白猪组织间的表达差异与生长发育相关,150和180日龄阶段,背部皮下脂肪组织中表达量极显著高于肝脏和背最长肌组织(P<0.01),初生、30日龄和90日龄阶段,背最长肌中的表达量极显著高于肝脏和背部皮下脂肪组织(初生阶段无脂肪组织样)(P<0.01);而马身猪整个发育过程中,背最长肌组织表现为优势组织,极显著高于其他2种组织(除120日龄阶段外)(P<0.01),脂肪组织表达量次之,肝脏组织中表达量较少。品种、日龄、组织及品种与日龄、组织与日龄的互作效应对FAS基因mRNA的相对表达量均有极显著影响(P<0.01)。FAS基因直接参与脂肪酸的合成,对猪肉质性状的遗传改良具有重要意义。The developmental expressions of fatty acid synthase（FAS）gene mRNA in liver,longissimus dorsi and backfat at seven phases（1,30,60,90,120,150 and 180-day old）of Mashen pig and Large White pig were studied by Real-time quantitative PCR,so that to discuss the spatial and temporal characteristics of FASgene expression and the relationship with fat metabolism.The results showed that in terms of inter-breeds comparison,the different expression of FAS gene mRNA in liver,longissimus dorsi and backfat of developmental phases of Mashen pig and Large White pig was significant or extremely significant（except in liver at 1-day old and in backfat at 120-day old）（P〈0.05;P〈0.01）.The developmental expressions of FASgene mRNA in three tissues of Large White pig were age-dependent,during the phases of 150-day and 180-day,the expressions of FASgene mRNA in backfat was significantly higher than those in liver and longissimus dorsi（P〈0.01）,and during the phases of 1-day,30-day and 90-day,the expressions of FASgene mRNA in longissimus dorsi were significantly higher than those in liver and backfat（no samples of 1-day in backfat）（P〈0.01）.While during the entire developmental phases of Mashen pig,longissimus dorsi was superiority tissue that the expression of the gene was significantly higher than those in other tissues,followed by the expression in backfat,and that in liver was the least.Breed,age,as well as the interactions of breed×age and tissue×age had significant effects on the relative expression of FASgene mRNA（P〈0.01）.FAS gene is important for the genetic improvement of meat quality traits of pig as it directly participating in the synthesis of fatty acids.

关 键 词：猪 脂肪酸合成酶基因mRNA 实时荧光定量PCR 发育性表达 

分 类 号：S828.2[农业科学—畜牧学]