不同因素对猪卵母细胞体外成熟培养的影响  

Effect of Different Factors on in Vitro Maturation of Porcine Oocytes

作　　者：林筱璐 林秀娇[1] 王秀爱 刘凤军[3] 庄益芬[1] 张兴锋[1] 李鸿翔[1] 洪志勇[1] 刘芳[1] 张文昌[1] 

机构地区：[1]福建农林大学动物科学学院,福建福州350002 [2]福清市永诚畜牧有限公司,福建福清350319 [3]河南科技大学动物科技学院,河南洛阳471003

出　　处：《中国畜牧兽医》2014年第10期205-209,共5页China Animal Husbandry & Veterinary Medicine

基　　金：福建省科技计划项目(2008N0001)

摘　　要：本试验旨在探讨不同外源激素组合的添加、胰岛素—转铁蛋白—亚硒酸钠(ITS)的添加、不同培养皿、石蜡油的添加对卵母细胞体外成熟培养(IVM)的影响。结果显示:1孕马血清促性腺激素+人绒毛膜促性腺激素+促卵泡素(PMSG+HCG+FSH)组高于促卵泡素+促黄体素(FSH+LH)组和尿促性腺激素(hMG)组,相互之间差异显著(80.1%、68.3%、53.3%,P<0.05);2添加1%ITS到猪卵母细胞培养液中对卵母细胞成熟率无显著提高(P>0.05),但显著提高了孤雌激活后孤雌胚胎的卵裂率和囊胚率(63.3%、55.1%,18.7%、12.1%,P<0.05);3凹槽皿组猪卵母细胞成熟率显著高于四孔板和30mm塑料皿组(73.3%、68.0%、68.3%,P<0.05);4石蜡油的添加对卵丘细胞扩展和卵母细胞体外成熟率均有显著提高(84.8%、69.9%,P<0.05)。结果表明培养液中添加PMSG+HCG+FSH和ITS及选用凹槽皿、成熟培养液上覆石蜡油可提高猪卵母细胞IVM效果。Effects of several factors such as exogenous hormones and ITS,culture methods,paraffin oil on in vitro maturation（IVM）for porcine oocytes were studied.The results indicated that,the group supplemented with PMSG＋HCG＋FSH was significantly higher than FSH＋LH and HMG in mature media with regard to the mature rates of porcine oocytes（80.1%vs 68.3%vs 53.3%,P〈0.05）;the mature rate of porcine oocytes cultured in mature media supplemented with 1%ITS was not improved,while the cleavage rate and blastocyst rate of parthenogenesis embryos were significantly increased（63.3% vs55.1%,18.7%vs 12.1%,P〈0.05）;the groove dish group was significantly higher than four-hole plate and 30 mm plastic dish group in terms of the mature rates of porcine oocytes（73.3%vs 68.0%vs 68.3%,P〈0.05）;mature media supplemented with paraffin oil was significantly increased the cumulus expansion and maturation rates for porcine oocytes（84.8% vs69.9%,P〈0.05）.The results showed that the mature rates of porcine oocytes cultured in groove dish,supplemented with paraffin oil,PMSG＋HCG＋FSH and ITS were significantly improved.

关 键 词：猪卵母细胞 体外成熟培养(IVM) 培养方式 石蜡油 外源激素 胰岛素—转铁蛋白—亚硒酸钠(ITS) 

分 类 号：S828[农业科学—畜牧学]