针刺不同穴位对寒凝证类痛经大鼠体表区域温度的影响  

Effects of needling different acupoints on skin temperature in SP6,SP10 and CV4 in the rat model of cold congealing and dysmenorrhea

作　　者：杨佳敏[1] 沈小雨[1] 罗丽 任晓暄[1] 郭孟玮[1] 赵雅芳[1] 张玲[1] 申松希[1] 齐丹丹[1] 朱世鹏[1] 许咏思[1] 嵇波[1] 张露芬[1] 李晓泓[1] 朱江[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京医学硕士研究生100029

出　　处：《医学研究生学报》2014年第9期900-904,共5页Journal of Medical Postgraduates

基　　金：国家重点基础研究发展计划(973计划)(2012CB518506);国家自然科学基金(30973793);教育部高等学校博士学科点专项科研基金(20090013110005)

摘　　要：目的：红外热成像技术可用于某些疾病的诊断和辅助诊断。文中旨在探讨针刺在调节不同经穴温度变化上的规律与差异，以及针刺是否引起经脉循行线上穴区温度的循经性升高或降低反应。方法将32只雌性SD大鼠按随机数字列表法分为等渗盐水组、寒凝证类痛经模型组（模型组）、三阴交组、关元组，每组8只。除等渗盐水组外，其余各组大鼠均连续10 d皮下注射苯甲酸雌二醇，末次给药1 h后，腹腔注射缩宫素2 U只/，将大鼠置于-25℃的低温冰柜中连续受冻5 d，每天4 h，制备寒凝证类痛经大鼠模型，等渗盐水组每日给予同等剂量的等渗盐水，不冷冻。运用红外热成像仪检测三阴交穴区、血海穴区、关元穴区的基础温度（针刺前），记录针刺即刻，针刺后5、10、20（取针）、30、40、50、60 min各时间点的温度。结果在即刻至针刺后5 min时，三阴交组及关元组左侧三阴交穴温度显著下降[（-0．56±0．22）℃ vs （-0．48±0．11）℃， P＜0．01]、右侧血海穴区体表温度显著下降[（-0．64±0．21）℃vs （-0．45±0．13）℃，P＜0．05]；在5～10 min时间段，三阴交组右侧三阴交穴区[（0．49±0．35）℃]、右侧血海穴区体表温度[（0．18±0．20）℃]均显著性升高（P＜0．01）；在20～30 min时间段，三阴交组右侧三阴交穴区体表温度较模型组和关元组显著升高[（0．14±0．25）℃，P＜0．01]；在30～40 min时，三阴交组右侧血海穴区体表温度较模型组显著升高[（-0．03±0．11）℃，P＜0．01]。各组左侧血海穴区、关元穴区差异无统计学意义（P＞0．05）。结论针刺三阴交、关元均可调节三阴交穴区、血海穴区的体表温度变化。三阴交组右侧三阴交穴区、血海穴区出现同时间段的循经性升温效应。说明针刺后相同经脉上穴区温度具有循经动态变化的特点。Objective Infrared thermal imaging can be applied to the diagnosis and auxiliary diagnosis of some diseases . The aim of this study is to explore acupuncture-induced changes in skin temperature in acupoint areas and whether skin temperature in -creases or decreases in the acupoint areas along meridians . Methods Thirty two female SD rats were randomly and equally divided into four groups：saline control,cold congealing and dysmenorrhea model , Sanyinjiao （SP6）, and Guanyuan（ CV4）.Models were es-tablished in the latter three groups by subcutaneous injection of estradiol benzoate at 0.5 mg for 10 successive days and , 1hour after the last administration , intraperitoneal injection of oxytocin at 2 U, followed by exposure of the rats to-25℃in a freezer 4 hours a day for 5 days.Meanwhile , the control rats received normal saline only and were not exposed to low temperature .Infrared thermal imaging was used to measure the skin temperature at the acupoint areas of SP6, Xuehai （SP10）, and CV4 before and at 0, 5, 10, 20, 30, 40, 50 and 60 min after needling . R esults At 0 to 5 min after nee-dling, the skin temperature of the left SP6 and right SP10 was signifi-cantly decreased in both the SP6 and CV4 groups （ [ -0. 56 ± 0.22]℃and [-0.48 ±0.11]℃, P〈0.01）, and so was that of the right SP10 （[ -0.64 ±0.21]℃ and [ -0.45 ±0.13]℃, P〈0.05）.At 5 to 10min, the skin temperature of the right SP6 and SP10 was markedly increased in the SP6 group （[-0.49 ±0.35]℃and [-0 .18 ±0.20]℃, P〈0.01）, and so was that of the right SP6 in the SP6 group at 20 to 30 min （[ -0.14 ±0.25]℃） as compared with the model and CV4 groups （P〈0.01）.At 30 to 40 min, the skin temperature of the right SP10 was remarkably elevat-ed in the SP6 group （[ -0.03 ±0.11]℃） in comparison with the model group （P〈0.01）.No significant differences were observed in the skin temperature of the left SP10 and CV4 at different time points among the four groups （P〉0.05）. Conclusion The skin tempera

关 键 词：穴区温度 循经性 红外热成像 寒凝证类痛经模型 

分 类 号：R246[医药卫生—针灸推拿学]