Spatial H202 Signaling Specificity： H202 from Chloroplasts and Peroxisomes Modulates the Plant Transcriptome Differentially  

作　　者：Nasser Sewelam Nils Jaspert Katrien Van Der Kelen Vanesa B. Tognetti Jessica Schmitz Henning Frerigmann Elia Stahl Frank Van Breusegem Veronica G. Maurino 

机构地区：[1]Institut of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universit~t, Universit~tsstraBe 1, 40225 D~sseldorf, Germany [2]Botany Department, Faculty of Science, Tanta University, 31527, Tanta, Egypt [3]Department of Plant Systems Biology, VIB, and Department of Plant Biotechnology and Bioinformatics, Ghent University,Technologiepark 927, B-9052, Gent, Belgium [4]Present address: Mendel Centre for Plant Genomics and Proteomics, CEITEC--Central European Institute of Technology, MasarykUniversity, Kamenice 5, CZ-62500 Brno, Czech Republic [5]Botanical Institute, Cologne Biocenter, University of Cologne, 50674 Cologne, Germany [6]Cluster of Excellence on Plant Sciences (CEPLAS), 40225 Dusseldorf and 50674 Cologne, Germany [7]Molecular Ecophysiology of Plants, Heinrich-Heine-Universitat, Universitatsstraf3e 1, 40225 D~sseldorf, Germany

出　　处：《Molecular Plant》2014年第7期1191-1210,共20页分子植物（英文版）

摘　　要：Hydrogen peroxide （H2O2） operates as a signaling molecule in eukaryotes, but the specificity of its signal- ing capacities remains largely unrevealed. Here, we analyzed whether a moderate production of H2O2 from two different plant cellular compartments has divergent effects on the plant transcriptome. Arabidopsis thaliana overexpressing glycolate oxidase in the chloroplast （Fahnenstich et al., 2008; Balazadeh et al., 2012） and plants deficient in peroxisomal catalase （Queval et al., 2007; Inze et al., 2012） were grown under non-photorespiratory conditions and then transferred to photorespiratory conditions to foster the production of H202 in both organelles. We show that H202 originating in a specific organelle induces two types of responses： one that integrates signals independently from the subcellular site of H202 production and another that is dependent on the H2O2 production site. H2O2 produced in peroxisomes induces transcripts involved in protein repair responses, while H2O2 produced in chloroplasts induces early signaling responses, including transcription factors and biosynthetic genes involved in production of secondary signaling messengers. There is a significant bias towards the induction of genes involved in responses to wounding and pathogen attack by chloroplas- tic-produced H202, including indolic glucosinolates-, camalexin-, and stigmasterol-biosynthetic genes. These transcriptional responses were accompanied by the accumulation of 4-methoxy-indol-3-ylmethyl glucosinolate and stigmasterol.Hydrogen peroxide （H2O2） operates as a signaling molecule in eukaryotes, but the specificity of its signal- ing capacities remains largely unrevealed. Here, we analyzed whether a moderate production of H2O2 from two different plant cellular compartments has divergent effects on the plant transcriptome. Arabidopsis thaliana overexpressing glycolate oxidase in the chloroplast （Fahnenstich et al., 2008; Balazadeh et al., 2012） and plants deficient in peroxisomal catalase （Queval et al., 2007; Inze et al., 2012） were grown under non-photorespiratory conditions and then transferred to photorespiratory conditions to foster the production of H202 in both organelles. We show that H202 originating in a specific organelle induces two types of responses： one that integrates signals independently from the subcellular site of H202 production and another that is dependent on the H2O2 production site. H2O2 produced in peroxisomes induces transcripts involved in protein repair responses, while H2O2 produced in chloroplasts induces early signaling responses, including transcription factors and biosynthetic genes involved in production of secondary signaling messengers. There is a significant bias towards the induction of genes involved in responses to wounding and pathogen attack by chloroplas- tic-produced H202, including indolic glucosinolates-, camalexin-, and stigmasterol-biosynthetic genes. These transcriptional responses were accompanied by the accumulation of 4-methoxy-indol-3-ylmethyl glucosinolate and stigmasterol.

关 键 词：hydrogen peroxide reactive oxygen species glycolate oxidase CATALASE PHOTORESPIRATION oxidative signaling. 

分 类 号：Q959.4[生物学—动物学] Q942