红凤菜新鲜茎叶中总黄酮提取物的LC-MS分析  

LC-MS analysis of total flavonoids extracts from fresh stem and leaf of Gynura bicolor

作　　者：任冰如[1] 吕寒[1] 陈剑[1] 梁呈元[1] 吴菊兰[1] 李维林[1] 

机构地区：[1]江苏省·中国科学院植物研究所(南京中山植物园)江苏省药用植物研究开发中心江苏省抗糖尿病药物筛选技术服务中心,江苏南京210014

出　　处：《植物资源与环境学报》2014年第3期108-110,共3页Journal of Plant Resources and Environment

基　　金：江苏省产学研联合创新资金项目(BY2012213);江苏省科技基础设施建设计划--科技公共服务平台项目(BM2011117)

摘　　要：红凤菜（Gynura bicolor DC.）为菊科（Compositae）菊三七属（Gynura Cass.）植物，别名血皮菜、观音菜、观音苋、紫背天葵等，主要分布于中国南方各地，全草均可入药[1]；也可作为蔬菜食用，在国内多个地区均有栽培和销售。目前已知红凤菜含有黄酮类、酚性酸类、萜类、甾醇类、脂肪酸类、生物碱类及花青素类等[2-7]成分。由于黄酮类成分多具有保护心血管、抗肿瘤、抗糖尿病、抗氧化、抗炎和抗病毒等作用[8]，因此对红凤菜中黄酮类成分的研究具有深度开发价值。Four total flavonoids extracts were obtained from fresh stem and leaf of Gynura bicolor DC. by method of ethanol extraction-macroporous resin column chromatography-polyamide column chromatography-recrystallization, and their compositions were analyzed by LC-MS technology. The results show that there are six groups of flavonoid absorption peak in total flavonoids extracts of G. bicolor, which is identified to be eight components including rutin, quercetin-3-O- rubinobioside, hyperoside, isoquercitrin, kaempferol-3-O-rubinobioside, kaempferol-3-O-rutinoside, kaempferol-3-O- galactoside and astragalin. This extraction method can effectively extract and purify total flavonoids from fresh stem and leaf of G. bicolor, so it can be used for factory production.

关 键 词：红凤菜 LC—MS 总黄酮 槲皮素 山柰酚 

分 类 号：Q946.83[生物学—植物学] R284.1[医药卫生—中药学]