原子力显微镜压痕曲线分析方法的比较研究  

Comparison of Cell Elasticity Analysis Methods Based on Atomic Force Microscopy Indentation

作　　者：王哲[1] 郝锋涛 陈晓虎[1] 杨周岐[1] 丁冲[1] 商澎[1] 

机构地区：[1]西北工业大学生命学院特殊环境生物物理学研究所空间生物实验模拟技术国防重点学科实验室,西安710072

出　　处：《生物医学工程学杂志》2014年第5期1075-1079,共5页Journal of Biomedical Engineering

基　　金：国家重点基础研究发展计划资助项目(2011CB710903);西北工业大学博士生论文创新基金资助项目(CX201021)

摘　　要：为了比较两种基于赫兹模型的原子力显微镜(AFM)力-距离分析方法,本研究使用AFM获取Hela和MCF-7两种细胞的力-距离曲线。在获得接触点的基础上,采用斜率法和两点法分别分析计算得到不同压痕深度下细胞的弹性模量。结果显示,Hela细胞的杨氏模量高于MCF-7细胞,这与两种细胞的微丝分布情况一致。研究还发现两种细胞的弹性模量随着压痕深度的增加而减少,但斜率法曲线的变化趋势平缓,提示斜率法能减少由于接触点判断错误引起的杨氏模量计算误差。本研究为AFM力-距离曲线分析方法的选择提供了参考依据。In order to investigate in greater detail the two methods based on Hertz model for analyzing force-distance curve obtained by atomic force microscopy,we acquired the force-distance curves of Hela and MCF-7cells by atomic force microscopy（AFM）indentation in this study.After the determination of contact point,Young＇s modulus in different indentation depth were calculated with two analysis methods of＂two point＂and＂slope fitting＂.The results showed that the Young＇s modulus of Hela cell was higher than that of MCF-7cell,which is in accordance with the Factin distribution of the two types of cell.We found that the Young＇s modulus of the cells was decreased with increasing indentation depth and the curve trends by＂slope fitting＂.This indicated that the＂slope fitting＂method could reduce the error caused by the miscalculation of contact point.The purpose of this study was to provide a guidance for researcher to choose an appropriate method for analyzing AFM indentation force-distance curve.

关 键 词：原子力显微镜 杨氏模量 接触点 力-距离曲线 

分 类 号：Q2-33[生物学—细胞生物学] Q-336