SD大鼠2型糖尿病模型的建立与评价  

Establishment and Evaluation of Type 2 Diabetes SD Rat Model

作　　者：邵俊伟[1] 蔡逊[1] 马丹丹[1] 胡逸林[1] 张翌[1] 

机构地区：[1]广州军区武汉总医院普通外科,湖北武汉430070

出　　处：《中国普外基础与临床杂志》2014年第10期1212-1215,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：湖北省自然科学基金(项目编号:2010CDB09201)~~

摘　　要：目的探讨高脂高糖喂养联合链脲佐菌素(streptozotocin,STZ)制备2型糖尿病大鼠模型的造模方法和影响因素。方法 60只6周龄SPF级雄性SD大鼠高脂高糖喂养4周后随机分为3组:对照组,20只,腹腔注射柠檬酸50 mg/kg,普通饲料喂养;糖尿病模型组,根据腹腔注射STZ剂量的不同分为2组,模型1组,20只,腹腔注射STZ 50 mg/kg,模型2组,20只,腹腔注射STZ 35 mg/kg,该2组大鼠继续高脂高糖喂养。分别在腹腔注射药物后第3、7、10及14天检测大鼠空腹血糖(fasting blood glucose,FBG)值,以14 d后血糖>16.7 mmol/L计算糖尿病成模率,并计算死亡率;实验结束时测定空腹血清胰岛素(fasting serum insulin,FSI)、甘油三脂(TG)和胆固醇(TC)水平。结果与对照组比较,两糖尿病模型组的TG、TC、FBG和FINS水平均明显升高(P<0.05);同时胰岛素敏感性(insulin sensetivity index,ISI)显著下降(P<0.05);模型2组的成模率高于模型1组(85%比75%),但死亡率低于模型1组(0比25%),P<0.05。结论高脂高糖喂养4周后,一次性腹腔注射35 mg/kg STZ制备的2型糖尿病大鼠模型成模率高,安全性强,模型稳定。Objective To evaluate the influencing factors and explore a better method of making rat model of type 2 diabetes by high fat and sugar diet and streptozotocin （STZ） injection. Methods Sixty SPF grade of 6 weeks male SD rats were fed with high fat and glucose diet by 4 weeks and then randomly divided into 3 groups, the control group rats （n=20） were injected citric acid by 50 mg/kg and fed with normal diet, and the diabetes mellitus group rats were further divided into 2 subgroups by the different doses of STZ： the rats of diabetes mellitus model group 1 （n=20） were injected by 50 mg/kg, while the rats of diabetes mellitus model group 2 （n=20） were injected by 35 mg/kg. The diabetes mellitus model group rats were fed with high fat and glucose diet continually. The fasting blood glucose （FBG） were measured on day 3, 7, 10, and 14, respectively. The success model rate （blood glucose 〉16.7 mmol/L after 14 days） and the mortality rate were calculated. Meanwhile fasting serum insulin level （FSI）, total serum cholesterol （TC）, and triglyceride （TG） were measured. Results Compared with the control group, the levels ofFBG, FSI, TC, and TG were increased significantly in the diabetes mellitus model group 1 and 2 （P〈 0.05）. And insulin sensitivity was worsen markedly （P〈0. 05）. But the diabetes mellitus model group 2 had higher success rate of making model （85% vs. 75%） and lower mortality （0 vs. 25%）, P〈 0. 05. Conclusion Rat model of type 2 diabetes induced by 4 weeks of high fat and sugar feeding and 35 mg/kg STZ injection has high morbidity, strongly security, and stable features.

关 键 词：2型糖尿病 大鼠模型 链脲佐菌素 高脂高糖饮食 

分 类 号：R587.1[医药卫生—内分泌]