胆汁酸影响兔肺泡灌洗液的表面张力  

Effect of bile acids on surface tension of bronchoalveolar lavage fluid in rabbits

作　　者：王斐[1] 赵丛[2] 田映红[1] 尹艳茹[1] 

机构地区：[1]南方医科大学基础医学院生理教研室,广东广州510515 [2]南方医科大学临床医学八年制2008级,广东广州510515

出　　处：《南方医科大学学报》2014年第10期1465-1468,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(31071010,31100806)~~

摘　　要：目的通过观察高胆汁酸血症状态下家兔支气管肺泡灌洗液表面张力的变化以及胆汁稀释液和5种不同游离胆汁酸在体外对家兔支气管肺泡灌洗液表面张力的影响,为认识胆汁酸调节呼吸功能提供实验依据。方法 30只新西兰雄性大白兔,使用0.9%生理盐水做支气管肺泡灌洗,肺泡灌洗液进行表面张力测定。实验一组测定高胆汁酸血症家兔动物模型肺泡灌洗液表面张力的变化;实验二组比较了生理盐水与不同稀释度胆汁液表面张力及对肺泡灌洗液表面张力的影响;实验三组比较了5种不同游离胆汁酸水溶液表面张力及对支气管肺泡灌洗液表面张力的影响。结果(1)高胆汁酸血症状态下家兔动物模型肺泡灌洗液表面张力与对照组相比明显升高(P<0.05);(2)支气管肺泡灌洗液中加入不同稀释度(1∶15、1∶10、1∶5)的胆汁稀释液,肺泡灌洗液表面张力就增加了21.15%、26.09%、19.64%(P<0.001);(3)5种游离胆汁酸水溶液中UDCA对支气管肺泡灌洗液表面张力没有明显影响(P>0.05),其他4种游离胆汁酸都可以升高支气管肺泡灌洗液表面张力(P<0.001),CDCA增加了16.10%,CA增加了21.66%,LCA增加了14.21%,DCA增加了13.05%。结论体内实验发现高胆汁酸血症状态下,肺泡灌洗液表面张力明显升高。体外实验进一步证明胆汁稀释液可以增加支气管肺泡灌洗液的表面张力;5种游离胆汁酸水溶液中除UDCA外其他4种游离胆汁酸都可以升高支气管肺泡灌洗液表面张力,说明这四种游离胆汁酸可能可以通过乳化肺泡表面活性物质,增加了肺泡表面张力,影响呼吸功能。Objective To observe changes in surface tension of bronchoalveolar lavage fluids（BALF） in rabbits with hyperbilirubinemia and the influence of bile diluents and 5 different bile acids on BALF surface tension to provide better insight into the regulatory role of bile acids on respiratory function. Methods Bronchoalveolar lavage with 0.9% normal saline was carried out in 30 male New Zealand rabbits and the surface tensions of BALF were measured. The changes in BALF surface tension was measured in rabbits with hyperbilirubinemia. Different concentrations of bile diluents, normal saline, or water solutions of 5 bile acids were added into the collected BALF to test their influence on the surface tension of BALF.Results The BALF from rabbits with hyperbilirubinemia showed a significantly increased surface tension（P0.05）. The bile diluents（1∶15, 1∶10, and 1∶5） added into the BALF increased the surface tension of the BALF by 21.15%, 26.09%, and 19.64%,respectively. Among the water solutions of the 5 bile acids, UDCA produced no significant influence on the surface tension of BALF while CDCA, CA, LCA, and DCA increased the surface tension by 16.10%, 21.66%, 14.21%, and 13.05%, respectively.Conclusion The surface tension of BALF increases significantly during hyperbilirubinemia. Bile diluents as well as the free bile acids CDCA, CA, LCA and DCA, but not UDCA, can increase the surface tension of BALF, suggesting that these bile acids may emulsify pulmonary alveolar surfactants to increase the alveolar surface tension.

关 键 词：胆汁 游离胆汁酸 肺泡灌洗液 表面活性物质 肺泡表面张力 

分 类 号：R332.2[医药卫生—人体生理学]