CRISP2基因及蛋白在弱精子症患者中的表达及其临床意义  

Expression of cysteine-rich secretory protein 2 in patients with asthenozoospermia and its clinical significance

作　　者：周俊豪[1] 薛康颐[1] 陈明坤[1] 周其赵[1] 杨建昆[1] 卞军[1] 李欣[2] 郭文彬[1] 夏慧[1] 亓涛[3] 刘存东[1] 

机构地区：[1]南方医科大学第三附属医院泌尿外科,广东广州510630 [2]南方医科大学肿瘤研究所,广东广州510515 [3]南方医科大学南方医院检验中心,广东广州510515

出　　处：《南方医科大学学报》2014年第10期1528-1533,共6页Journal of Southern Medical University

基　　金：广东省自然科学基金(S2012010009126)

摘　　要：目的弱精子症作为男性不育的重要因素之一,影响着全球15%的育龄夫妇,但其发病机制尚待阐明。课题组前期研究通过基因芯片技术发现CRISP2在弱精子症患者精子中表达明显下调,但其临床意义仍有待进一步阐明。方法收集2013年1月至2013年6月的弱精子症患者及健康男性精液标本各24例,检测这些精液样本中CRISP2基因及蛋白表达水平,探讨其与精子形态、运动能力及生育预后的相关性。结果弱精子症患者精液标本中CRISP2蛋白表达水平明显低于正常对照组,而CRISP2基因mRNA表达水平并无明显统计学差异。相关性分析显示CRISP2蛋白表达水平与形态正常精子率(r=0.6182,P=0.0037)及前向运动能力(r=0.6309,P=0.0029)呈明显正相关。经过密切随访,CRISP2蛋白高表达患者配偶受孕率明显高于CRISP2蛋白低表达患者(80.0%vs 20.0%,P=0.0230)。结论 CRISP2蛋白表达水平与弱精子症患者的精子形态、前向运动能力呈正相关,且CRISP2蛋白低表达的弱精子症患者生育预后不良,提示CRISP2作为弱精子症患者治疗新靶点的潜在价值。Objective To investigate the expression of cysteine-rich secretory protein 2（CRISP2） in spermatozoa of patients with asthenozoospermia and explore its clinical significance. Methods Semen samples were collected from 24 normal volunteers and 24 patients with asthenozoospermia for detecting CRISP2 mRNA and protein expressions using qRT- PCR and Western blotting, respectively. The correlation of CRISP2 expressions with sperm morphology, progressive motility and fertility prognosis were analyzed in patients with asthenozoospermia. Results CRISP2 protein expression was obviously lowered in the ejaculated spermatozoa of patients with asthenozoospermia as compared to the normal volunteers, but no significant difference in CRISP2 mRNA expression was found between the two groups. Correlation analysis showed that CRISP2 protein expression was positively correlated with normal sperm morphology（r=0.6182, P=0.0037） and progressive motility（r=0.6309, P=0.0029）. Follow- up study of the patients revealed a higher fertility rate in patients with a relatively high CRISP2 protein expression than in those with low CRISP2 protein expression（80.0% vs 20.0%, P=0.0230）. Conclusion The expression level of CRISP2 protein is positively correlated with normal sperm morphology and progressive motility. A reduced CRISP2 protein expression indicates poor fertility prognosis of patients with asthenozoospermia, suggesting the potential value of CRISP2 as a novel therapeutic target for treating asthenozoospermia.

关 键 词：弱精子症 CRISP2 精子形态 精子前向运动能力 生育预后 

分 类 号：R698.2[医药卫生—泌尿科学]