胃转流术对2型糖尿病大鼠胰高血糖素样肽1及小肠黏膜的影响  

The mechanism of glucagon-like peptide-1 on hypoglycemic effect of diabetic type 2 rats treated by gastric bypass operation

作　　者：任泽强[1] 章红[2] 张蓬波[1] 黄智龙 张秀忠[1] 陈守坤[1] 

机构地区：[1]徐州医学院附属医院普外科,江苏221002 [2]徐州医学院附属医院手术室,江苏221002 [3]兰陵县人民医院普外科

出　　处：《中华实验外科杂志》2014年第10期2172-2174,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省徐州市科技计划项目（XM12B028）

摘　　要：目的 观察胃转流术（GBP）对2型糖尿病大鼠胰高血糖素样肽1（GLP-1）及小肠黏膜的影响,探讨GBP术降糖机制.方法 健康雄性SD大鼠随机分为正常手术组（NO组）、正常对照组（NC组）、糖尿病手术组（DO组）、糖尿病对照组（DC组）.术前及术后第1、2、4、8周分别检测各组GLP-1、空腹血糖（FPG）、胰岛素.术后2个月苏木素-伊红（HE）染色观察末段回肠黏膜病理变化,测量肠黏膜厚度和绒毛高度.结果 DO、NO组GBP术后第8周空腹GLP-1分别由术前的（7.38±1.71） pmol/L和（7.23±1.59） pmol/L升高到（17.80±1.39） pmol/L和（15.48±1.21） pmol/L（P＜0.05）.DO组术后第8周FPG由术前的（17.71 ±1.99） mmol/L下降到（5.95 ±0.51） mmol/L （P＜0.05）.术后各时相点DO组GLP-1、FPG明显低于相应时间点DC组（P＜0.05）,NO组手术前后FPG无明显变化.DO组术后第8周胰岛素由术前的（11.93±0.75） mmol/L升高到（14.80±0.78） mmol/L（P＜0.05）.DO、NO组肠黏膜厚度和绒毛高度均显著高于DC组和NC组（P＜0.05）.结论 GBP术后肠黏膜的增生导致GLP-1升高可能为降糖机制之一.Objective To observe the and influence on intestinal mucosa on type 2 diabetes rats and to research the function of glucagon-like peptide-1 （GLP-1）.Methods Healthy male SD rats were randomly divided into normal operation group （NO）,normal control group （NC）,diabetic operation group （DO） and diabetic control group （DC）.The GLP-1,plasma glucose and insulin were tested preoperatively and 1,2,4,8 weeks postoperatively.Intestinal mucosa were examined by hematoxylin and eosin （HE） stain 2 months after operation.The mucosal thickness and villus height were measured by histological analysis.Results The DO group＆#39; s and NO group＆#39; s GLP-1 increased from （7.38 ± 1.71） pmol/L and （7.23 ± 1.59） pmol/L to （17.80 ± 1.39） pmol/L and （15.48 ± 1.21） pmol/L （P 〈 0.05） at 8th week after gastric bypass operation respectively.The GLP-1 level of DO group was higher than that of DC and NC groups at corresponding time （P 〈 0.05）.The DO group＆#39; s fasting plasma glucose level was decreased which from （17.71 ± 1.99） mmol/L （preoperatively） to （5.95 ± 0.51） mmol/L （8 weeks postoperative） （P 〈 0.05）.The plasma glucose of DC group was higher than DO group at corresponding time （P 〈0.05）.The DO group＆#39; s fasting plasma insulin increased from the preoperative （11.93 ± 0.75） mmol/L to （14.80 ± 0.78） mmol/L （P 〈 0.05）at 8th week after gastric bypass operation respectively.Postoperative pathologic exam showed that the mucosal thickness and villus height in DO group and NO group were significantly higher than DC group and NC group.Conclusion The glucagons-like peptide-1 plays an important role on hypoglycemic effect of diabetic type 2 rats treated by gastric bypass operation.

关 键 词：胃转流术 胰高血糖素样肽1 2型糖尿病 血糖 小肠黏膜 

分 类 号：R587.1[医药卫生—内分泌]