灯盏花素对大鼠液压冲击脑损伤的神经保护作用及其机制  

Effect and its mechanism of breviscapine on nervous function score of fluid percussion injury in rats

作　　者：孙国柱[1] 杨建凯[1] 李胜超[2] 赵宗茂[1] 马波涛[1] 朱小辉[1] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000 [2]河北医科大学第四医院肝胆外科

出　　处：《中华实验外科杂志》2014年第10期2191-2193,共3页Chinese Journal of Experimental Surgery

基　　金：河北省自然科学基金资助项目（C2008001084、H2014206383）;河北省中医药局课题项目（2013014）;河北省医学科学重点课题项目（20130178）

摘　　要：目的 观察灯盏花素对大鼠液压冲击脑损伤神经保护作用并探讨其机制.方法 成年雄性SD大鼠80只制作颅脑损伤模型,随机分为两组：模型组和干预组.干预组应用灯盏花素90 mg/kg腹腔注射.两组大鼠均于术后6、12、24 h、3、7d进行神经功能评分和组织学观察,并用干湿重法、免疫组织化学法和Western blot分别测定水肿脑组织含水量及其水通道蛋白4（AQP4）的表达变化.结果 与模型组比较,干预组大鼠神经损伤明显缓解,脑组织水肿程度减轻,以24 h、3d减轻最为明显[24 h：（82.54±1.89）％比（80.84±1.08）％;3d：（80.61±0.49）％比（79.11 ±1.14）％];神经功能评分明显改善,以24 h、3、7d3个时间点最为显著（24 h：5.83 ±0.41比7.33 ±0.82;3d：6.17 ±0.75比7.50 ±0.55;7 d：7.00±0.63比8.33 ±0.52）,同时AQP4蛋白表达也出现不同程度减少,尤以24 h、3、7d显著（24 h：0.83 ±0.03比0.57 ±0.04;3 d：0.91 ±0.02比0.61 ±0.02;7d：0.64±0.05比0.42±0.07）.结论 灯盏花素能够改善神经细胞受损程度,通过下调AQP4表达减轻液压冲击脑损伤脑水肿发挥神经保护作用.Objective To investigate the effect of breviscapine on neuroprotection of rats following fluid percussion injury.Methods Eighty rats were employed to establish experimental models according to Dixon＆#39; s method and randomly divided into two group：model group and intervention group.The rats in intervention group were adminstered breviscapine （90 mg/kg） by intraperitoneal injection.Nervous function score,brain water content,histological changes,and aquaporin 4 （AQP4） expression were observed by Shapira and Wahld method,dry-wet measurement,light microscopy,immunohistochemistry and Western blotting at 6,12,24 h and 3,7 d after strike respectively.Results As compared with model group,the pathological changes were significantly alleviated,and water content of edemamtous brain was significantly reduced in intervention group,especially at 24 h and 3 d after strike [24 h：（82.54 ± 1.89） % vs.（80.84±1.08）%; 3 d：（80.61 ±0.49）% vs.（79.11 ±1.14）%].Nervous function score was increased significantly,especially at 24 h,3 d and 7 d after strike （24 h：5.83 ±0.41 vs.7.33 ±0.82;3 d：6.17 ±0.75 vs.7.50 ±0.55; 7 d：7.00 ±0.63 vs.8.33 ±0.52）.AQP4 protein expression was decreased signifacantly at 24 h,3 d and 7 d after strock （24 h：0.83 ± 0.03 vs.0.57 ± 0.04; 3 d：0.91 ± 0.02 vs.0.61 ±0.02; 7 d：0.64 ±0.05 vs.0.42±0.07）.Conclusion Breviscapine plays arole of neuroprotective effect on brain damage from fluid percussion injury by downregulating AQP4 expression and improving nervous cell injury.

关 键 词：灯盏花素 脑损伤 液压冲击 水通道蛋白4 脑水肿 

分 类 号：R651.15[医药卫生—外科学]