沙漠干热环境创伤失血性休克大鼠血清炎性因子和肺组织环氧化物酶-2的变化  

The changes of serum inflammatory cytokines and cyclooxygenase-2 mRNA in lung tissue and their role in secondary lung injury of traumatic hemorrhagic shock rats in dry heat enviroment of desert

作　　者：李瑞[1] 刘江伟[1] 钱建辉[1] 钱若筠[1] 张琼[1] 杨向新[1] 

机构地区：[1]兰州军区乌鲁木齐总医院肝胆外科,乌鲁木齐830000

出　　处：《中华实验外科杂志》2014年第10期2211-2213,共3页Chinese Journal of Experimental Surgery

基　　金：军队临床高新技术重大项目（2010gxjs016）

摘　　要：目的 探讨沙漠干热环境创伤失血性休克大鼠血清白细胞介素-1β（IL-1β）、IL-6和肺组织环氧化酶-2（COX-2）mRNA的变化与肺脏继发性损伤的关系及其机制.方法 将140只雄性SD大鼠随机分为干热环境和常温环境创伤失血性休克组,每组又分别设对照组、休克后0、0.5、1.0、1.5、2.0、3.0组7个亚组,每组10只,建立创伤失血性休克模型后,留取血清和肺组织,观察肺组织病理学改变,酶联免疫分析（ELISA）法检测血清IL-1β、IL-6浓度及实时荧光定量聚合酶链反应（FQ-PCR）检测肺组织COX-2 mRNA表达量.结果 干热组各时间点肺组织病理学评分较高,干热组休克后各时间点血清IL-1β、IL-6及肺组织COX-2 mRNA表达量（3.46±0.19）均高于常温组（2.13±0.13）,干热组血清IL-1β、COX-2 mRNA峰值出现在休克后1.5h,常温组峰值则出现在2.0h,而干热组与常温组IL-6峰值出现均较IL-1β推迟,血清IL-1β、IL-6及COX-2 mRNA表达与肺损伤病理学评分呈正相关.结论 在沙漠干热环境中创伤失血性休克继发性肺损伤时,肺脏损伤较常温环境下严重、损伤提前,血清IL-1 β、IL-6、COX-2在沙漠干热环境创伤失血性休克继发性肺损伤过程中可能起重要作用.Objective To study the changes of serum infflammatory cytokines cell interleukin-1 β （IL-1 β）,interleukin-6 （IL-6） and cyclooxygenase-2 （COX-2） mRNA in lung tissues and their role in secondary lung injury of traumatic hemorrhagic shock rats in dry heat environment of desert.Methods 140 male SD rats were randomly divided into the dry heat environment and the normal temperature environment traumatic hemorrhagic shock group,respectively,each groups were randomly divided into 7 subgroup：the control group,after shock 0,0.5,1.0,1.5,2.0,3.0 h group,10 rats in each subgroup,After the establishment of traumatic hemorrhagic shock rat models in each environment,at the corresponding time points,serum and lung tissue were harvested.Pathological changes of lung tissue were observed,serum IL-1 β,IL-6 concentrations was detected with the method of enzyme-linked immunoassay （ELISA）,and expression of COX-2 mRNA of lung tissue was detected by Real-time quantitative polymerase chain reaction.Results The dry heat envionment group has a higher lung histopathological injury score.The serum IL-1 β and expression of COX-2 mRNA （3.46 ± 0.19） in lung tissue in dry heat environment group were higher than that of the normal temperature environment group at each time point （2.13 ± 0.13）,of which the peak appeared at 1.5 h in dry heat environment group,while the peak appeared at 2 h in the normal temperture environment group,the IL-6 peak appeared later than the IL-1 β in both environment groups.The concentration of serum IL-1 β,IL-6 and expression of COX-2 mRNA in lung tissue were positively correlated with pathological lung injury score in both environment groups （P 〈 0.05）.Conclusion The secondary lung injury of traumatic hemorrhagic shock was more serious and appead eralier in dry heat environment group than that of the normal temperature.The serum IL-1 β,IL-6 and the expression COX-2 may play and important role in the secondary lung injury of the traumatic hemorrhagic shock rats in the dry heat env

关 键 词：沙漠 干热环境 创伤失血性休克 肺损伤 白细胞介素-1Β 白细胞介素-6 环氧化酶-2 

分 类 号：R641[医药卫生—外科学]