纳米壳聚糖骨形成蛋白水凝胶修复下颌骨缺损实验研究  

The effects of nano-chitosan /bone morphogenetic protein gel on the repair of mandibular defect

作　　者：陈玉阳[1,2] 谢富强[3] 张赟[1] 王新[1] 孙健[3] 

机构地区：[1]兰州大学口腔医学院,730000 [2]武威市人民医院 [3]兰州大学第二医院口腔科

出　　处：《实用口腔医学杂志》2014年第5期624-628,共5页Journal of Practical Stomatology

基　　金：甘肃省卫生行业科研计划资助(编号:GSWST2011-09);兰州市科技计划项目(编号:2011-2-50)

摘　　要：目的:观察可注射性纳米壳聚糖骨形成蛋白水凝胶修复颌骨缺损的效果。方法:以纳米壳聚糖为载体,加入重组人骨形成蛋白-2制备成可注射性纳米壳聚糖骨形成蛋白水凝胶(NCS/BMP-2 Gel)。取9只SD大鼠,在双侧背部皮下分别注射200μl(100μg/ml)NCS/BMP-2 Gel及单纯纳米壳聚糖水凝胶(NCS Gel)。于术后10、20、30 d分别处死3只,取材进行大体及常规病理学观察;选取54只SD大鼠,随机均分为3组(n=18)制备下颌骨缺损模型,实验组骨缺损中注入NCS/BMP-2Gel、对照组注入NCS Gel、空白组不注射。术后3、6、9周各组处死6只大鼠。分离下颌骨,用钼靶扫描图像,Image Pro-Plus软件比较其颌骨缺损面积;常规病理观察颌骨成骨情况。结果:实验侧、对照侧均形成皮下结节,实验侧30 d结节内可见类骨样结构;钼靶扫描检查:植入后3、6、9周时,实验组剩余骨缺损面积均低于对照组及空白组(P<0.05);组织学观察显示,实验组下颌骨成骨情况优于对照组及空白组。结论:NCS/BMP-2水凝胶有良好的生物相容性,能促进颌骨缺损修复。Objective：To investigate the effects of Nano-chitosan rhBMP-2 Gel（NCS/BMP-2 Gel）on the repair of mandibular de-fect.Methods：NCS/BMP-2 Gel and NCS Gel were prepared and respectively injected into the subcutaneous space on both sides of dorsum of 9 rats.3 rats were respectively sacrificed 10,20 and 30 d after injection.The subcutaneous nodules were histologicaly ex-amined.Mandibular defect was made in 54 SD rats and the rats were divided randomly and into 3 groups（n=18）：NCS/BMP-2 Gel was implanted into the defects in group 1,NCS Gel in group 2,no injection in group 3.Animals were sacrificed 3,6 and 9 weeks after transplantation.X-ray examination,pathologic observation were conducted.Results：Subcutaneous nodules were found in both sides of the rat dorsum.The residual mandibular defect area in study group 1 was apparently smaller than that in group 2 and 3（P〈0.05）. More new bone formation was observed in the gel injected area in group 1 than in group 2 and 3.Conclusion：Nano-CS/BMP-2 Gel is biocompatible and can accelerate the healing of mandibular defect.

关 键 词：颌骨缺损 纳米壳聚糖 水凝胶 骨形成蛋白-2 

分 类 号：R318.08[医药卫生—生物医学工程]