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机构地区:[1]襄阳市中心医院口腔科,441021 [2]第四军医大学口腔医学院修复科 [3]第四军医大学口腔医学院种植科
出 处:《实用口腔医学杂志》2014年第5期641-644,共4页Journal of Practical Stomatology
摘 要:目的:研究微弧氧化纯钛表面对MC3T3-E1细胞胶原分泌和成骨相关基因表达的影响。方法:纯钛圆片分为2个组:微弧氧化组(MAO)和抛光组(PT),培养板表面(TC)作为对照组。用场发射扫描电镜(FESEM)观察试样表面形态,表面粗糙度仪测量其表面粗糙度。将细胞接种于3组材料表面体外培养,培养第12天用粘胶纤维红染色检测细胞胶原分泌,第16天RT-PCR检测细胞成骨相关基因表达。结果:纯钛表面经微弧氧化处理后形成一层多孔氧化层,表面粗糙度增加(P<0.01)。细胞在MAO组试件表面的胶原分泌高于PT或TC组表面(P<0.05)。细胞在MAO组试件表面OSX、COL-Iα1及OPN基因的表达均稍高于PT表面。结论:相比于纯钛抛光表面,微弧氧化纯钛表面更能促进MC3T3-E1细胞胶原分泌。Objective:To investigate the effects of titanium surface modified by micro-arc oxidation (MAO)on the collagen secre-tion and the expression of osteogenesis-related genes of MC3T3-E1 cells.Methods:Pure titanium discs were divided into two groups:Titanium with modified surface by MAO(MAO group)and titanium with polished surface(PT group).Tissue culture polysty-rene plates (TC)were used as controls.Surface morphology of the samples was examined by field-emission scanning electron micros-copy(FESEM).Surface roughness of samples was measured by surface roughness meter.MC3T3-E1 cells were cultured on the sur-face of the samples and the collagen secretion on the samples was measured by sirius red-based colorimetric microassay after 12 days of culture.On day 16 of culture,the expression of osteogenesis-related genes was examined by RT-PCR.Results:A porous oxide layer was observed on the surface of MAO treated samples and the surface roughness was more than that of PT group(P〈0.01 ). Cells in MAO group showed higher collagen secretion than in PT or TC groups(P〈0.05).MAO treated surface induced higher OSX, COL-Iα1 and OPN expression than PT.Conclusion:MAO treated titanium surface may induce more collagen secretion of MC3T3-E1 cells than PT.
分 类 号:R318.08[医药卫生—生物医学工程] Q78[医药卫生—基础医学]
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