分子标记辅助选育聚合抗稻瘟病基因和抗白叶枯病基因的水稻改良新恢复系  

Developing Improved Lines of Three Rice Restorers Pyramided Resistant Genes to Blast and Bacterial Blight by Marker-assisted Selection

作　　者：田大刚[1,2] 陈在杰[2] 陈子强[2] 林艳[2] 周元昌[1] 陈松彪[2] 王锋[1,2] 

机构地区：[1]福建农林大学、作物遗传育种与综合利用教育部重点实验室,福州350002 [2]福建省农业科学院生物技术研究所、福建省农业遗传工程重点实验室,福州350003

出　　处：《分子植物育种》2014年第5期843-852,共10页Molecular Plant Breeding

基　　金：福建省农业科学院院青年基金项目(2012DQA-8);福建省自然科学基金杰出青年基金项目(2011J06009);福建省农业科学院引进海外人才科研启动基金项目(HWRC2011-01)共同资助

摘　　要：本研究选择三个新育成杂交水稻恢复系闽恢3189、闽恢3229和闽恢6118为受体亲本,以携带抗稻瘟病主基因Pi9的C750和抗白叶枯病主基因Xa23的C682为供体亲本,利用分子标记辅助选择和田间鉴定选择相结合方法,经过多代回交、自交,获得8个导入Pi9或Xa23或Pi9+Xa23的水稻恢复系分子改良系。以19个福建近年稻瘟菌优势菌株和一个白叶枯病强致病菌株P6进行人工接种,结果表明,Pi9或Xa23的导入明显提高了水稻恢复系分子改良系的稻瘟病或白叶枯病抗性。分子改良系及其杂交组合的有效穗数、千粒重和单株产量等与受体亲本及其杂交组合的相关农艺性状无明显差异。这些抗性分子改良系有望升级原有受体亲本,在生产中具有良好应用前景。A broad-spectrum blast resistance gene P/9 from a donor line C750, and a bacterial blight （BB） resis- tance gene Xa23 from a donor line C682 were introgressed into three novel Indica rice restorer lines Minhui 3229, Minhui 3189, and Minhui 6118 by marker-assisted backcrossing in combination with stringent phenotypic selections. A total of eight genetic improved lines carrying Pi9, Xa23 or Pi9＋Xa23 were developed. Inoculations of the developed lines carrying Pi9 with 19 representative blast isolates collected from Fujian province, and of the developed lines carrying Xa23 with a destructive Xanthomonas oryzae pv. oryzae strain PXO99 （P6） revealed that the improved rice restorers conferred significantly enhanced resistance to blast, or to BB. Investigation of major agronomic characteristics indicated that the improved lines and their derived hybrid combinations were almost identical to their original receptor lines and combinations for important yield traits, especially for productive panicles number/plant, 1 000-grain weight, and single-plant yield. Thus, the improvement of resistances to blast/ BB would facilitate the application of the newly bred restorer lines in agriculture production.

关 键 词：水稻恢复系 稻瘟病 白叶枯病 分子标记辅助选择 Pi9/Xa23 

分 类 号：S511[农业科学—作物学]