幽门螺杆菌侵袭细胞刺激AID表达  

Invasion of Helicobacter pylori induced AID expression

作　　者：张晓艳[1] 林允斌 张杰[1] 陈豪[1] 佘菲菲[1] 

机构地区：[1]福建医科大学基础医学院,消化道恶性肿瘤教育部重点实验室,福州350108

出　　处：《中国人兽共患病学报》2014年第9期923-928,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.81271784);福建省自然科学基金(No.2012J01130)~~

摘　　要：目的探讨幽门螺杆菌(Hp)侵袭内化对细胞AID表达的影响及相关机制。方法选择不同侵袭力的Hp,分别感染AGS细胞,以抗β1整合素抗体抑制侵袭为对照组,经庆大霉素保护性侵袭实验,用real-time RT-PCR和细胞免疫荧光分别检测AID mRNA和蛋白表达水平。用SN-50抑制AGS细胞NF-κB活性,同法检测Hp对AID表达的影响。结果高侵袭力的Hp诱导的AID蛋白和mRNA的表达量均高于低侵袭力的Hp,P<0.05;抗β1整合素抗体处理后各组AID蛋白表达均减少(P<0.05),高侵袭力组mRNA的表达也均减少(P<0.05),低侵袭力组mRNA的表达呈下降趋势;SN-50处理后各组AGS细胞AID蛋白和mRNA表达水平均降低,P<0.05。结论侵入胞内的Hp可刺激AGS细胞诱导AID的异常表达,这种诱导涉及NF-κB信号通路,细菌的诱导能力与内化细菌的数量呈正相关。Hp内化诱导宿主细胞异常表达AID可能是导致Hp相关肿瘤发生的机制之一。We investigated whether the internalized Helicobacter pylori （Hp） could induce AID expression and explored the corresponding mechanism in this study. Hp strains with different invasiveness invaded AGS cells, respectively. By anti-βl- integrin antibody, the invasion was inhibited as a control. We conducted the gentamiein protection assay, and then we detected the protein and mRNA expression of AID induced by the internalized Hp using immunofluorescence and real-time RT-PCR. U- sing SN-50 to inhibit NF-κB, we detected AID expression by the same ways. The protein and mRNA expression of AID in- duced by high invasiveness strains were found to be stronger than those induced by low invasiveness strains （P〈0.05）. After AGS cells were pretreated with anti-β1-integrin antibody, the protein expression of AID declined in each groups （P〈0.05）; the mRNA declined in high invasiveness groups （P〈0.05） and that was on a declining curve in low invasiveness groups. After AGS cells were pretreated with SN-50, the protein and mRNA expression of AID declined in each groups （P〈0.05）. It is evi- dent that the internalized Hp can induce AID aberrant expression, which may principally involve in NF-κB signal pathway. This induction and amount of the intracellular Hp have positive correlation. AID aberrant expression induced by internalized Hp may contribute to the development of Hp associated neoplasms.

关 键 词：幽门螺杆菌 侵袭 AID NF-ΚB 

分 类 号：R378[医药卫生—病原生物学]