熊果酸对ox-LDL诱导的人脐静脉内皮细胞氧化损伤的保护作用  

Protective effects of ursolic acid on ox-LDL induced oxidative damage in human umbilical vein endothelial cells

作　　者：郝冉冉[1] 王晨静[2] 仲伟珍[1] 夏蕴秋[1] 韩彦弢[1] 

机构地区：[1]青岛大学医学院,青岛266071 [2]青岛大学医学院附属医院,青岛266071

出　　处：《中药药理与临床》2014年第4期29-32,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然基金(No 81173593)

摘　　要：目的：复制氧化性低密度脂蛋白（oxidized low density lipoprotein,ox-LDL）诱导的人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cells,HUVECs）氧化损伤模型,并探讨熊果酸（Ursolic Acid,UA）对其保护作用。方法：以体外培养的HUVECs为实验对象,采用不同浓度（5,10,15,20μM）熊果酸及阳性对照药物维生素E预处理细胞16 h,再加入100μg/ml的ox-LDL培养24 h造成细胞氧化损伤。CCK-8检测细胞存活率,酶联免疫法检测各组细胞培养上清液过氧化氢（H2O2）、一氧化氮（NO）、丙二醛（MDA）含量,超氧化物歧化酶（SOD）活力以及各组细胞裂解液中一氧化氮合成酶（NOS）活力;采用SPSS 17.0软件对实验数据进行统计学分析。结果：与正常组相比,模型组细胞存活率、NO、SOD、NOS水平明显降低,MDA、H2O2水平明显增加;与模型组比较,熊果酸在5-20μM范围内,呈剂量依赖性提高细胞存活率,上调NO、SOD、NOS水平,并下调MDA、H2O2水平。结论：100μg/ml的ox-LDL能够诱导HUVECs发生氧化损伤,熊果酸能够通过抗氧化发挥保护作用,其机制可能与维持NOS、SOD活性、减少MDA、H2O2生成等有关。To investigate the protective effects and mechanisms of ursolic acid on damaged umbilical vein endothelial cells （HUVECs） induced by ox-LDL. Methods： HUVECs were administered with different concentrations（5,10,15,20 μM） of UA for 16 h. Then they were treated with 100 ？ g/ml ox-LDL for 24 h to duplicate an oxidative injury model. The cell viability of HUVECs was detected by CCK-8. The content of hydrogen peroxide （ H2O2 ）, nitric oxide （ NO）, malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） and ni- tric oxide synthase （NOS） were detected by enzymic method. The experimental data was statistical analysised by SPSS 17.0. Results： Com- pared with the control group, the cell viability, NO, SOD, NOS levels in model group were significantly declined while the MDA, H2 O2 lev- els were significantly increased （ P 〈 0.05）. Compared with the model group, UA could improve the cell viability, increase the NO, SOD, NOS levels and lower the MDA, H2 O2 levels in a dose-dependent manner. Conclusion： 100μg/ml ox-LDL could induce oxidative damage of HUVECs, and UA could play a protective role through its anti-oxidative effect. This may be related to its effect of maintaining NOS, SOD ac- tivity, reducing MDA, H2O2 generation and so on.

关 键 词：熊果酸 氧化性低密度脂蛋白 氧化损伤 一氧化氮合成酶 

分 类 号：R285[医药卫生—中药学]