黄药子与甘草配伍对大鼠肝CYP450酶活性及mRNA表达的影响  

Effects of Radix Glycyrrhizae compatibility of Rhizoma dioscorea bulbifera on the activity and expression of CYP450 enzymes in rat liver

作　　者：华碧春[1] 黄智锋[1] 刘娇[1] 程心玲[2] 陈小峰[3] 王英豪[1] 卓实[2] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]福建省人民医院,福州350004 [3]福建省第二人民医院,福州350003

出　　处：《中药药理与临床》2014年第4期79-81,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助课题(81274105)

摘　　要：目的:研究甘草减轻黄药子肝损伤对CYP450酶活性及基因表达的影响。方法:采用紫外分光光度法测定大鼠肝微粒体细胞色素P450、细胞色素b5含量及氨基比林N-脱甲基酶、红霉素N-脱甲基酶活性,采用RT-PCR技术检测大鼠肝脏CYP2E1、CYP3A4的mRNA的表达。结果:黄药子甘草1∶2配伍组能显著减轻黄药子致大鼠肝损害,与黄药子甘草1∶2配伍组(27 g/kg)比较,黄药子组(9 g/kg)肝微粒体蛋白浓度、P450含量、b5含量均明显下降;氨基比林N-脱甲基酶活性、红霉素N-脱甲基酶活性亦明显下降。在mRNA水平上,黄药子组9 g/kg显著诱导CYP2E1、CYP3A4基因的表达,与甘草1∶2配伍后(27 g/kg)表达显著降低。结论:甘草可能通过提高CYP450酶活性,抑制CYP2E1、CYP3A4的mRNA表达,对黄药子致肝损伤具有一定的保护作用。To study the effects of Radix Glycyrrhizae compatibility of Rhizoma dioscorea bulbifera on the activity and expression of CYP450 enzymes in rat livers. Methods： The contents of liver micrisomal cytochrome P450, cytochrome b5 and the activities of aminopyrine N-demethylase and erythromycin N-demethylase were examined by ultraviolet spectrophotometry. The mRNA levers of the expression of the CYP2E1 and CYP3A4 gene of the rat liver were assayed with RT-PCR. Results： The group of Rhizoma dioscorea bulbifera （9 g/kg） obvi- ously decreased the contents of liver micrisomal cytochrome P450, cytochrome b5 and the activities of aminopyrine N-demethylase and eryth- romyein N-demethylase. The mRNA levels of CYP2E1 and CYP3A4 were induced significantly by group of Rhizoma dioseorea bulbifera （9 g/ kg） after medication for 28 days separately and reduced after compatibility of Radix Glyeyrrhizae （27 g/kg）. Conclusion： The activity of CYP450 enzymes were induced and the mRNA expression were inhibited by Radix Glyeyrrhizae to protect liver injury which caused by Rhizo- ma dioseorea bulbifera.

关 键 词：黄药子 甘草 CYP450 基因表达 配伍减毒 

分 类 号：R285.5[医药卫生—中药学]