乌芩健脑方对痴呆模型大鼠海马CDK5和GSK-3表达的影响  

Study on effects of Wuqinjiannao Fang on expression of CDK5 and GSK-3 of Alzheimer's disease rats

作　　者：代渊[1] 杨涵[1] 王飞[1] 

机构地区：[1]成都中医药大学,成都610072

出　　处：《中药药理与临床》2014年第4期98-102,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金："重大新药创制"科技重大专项(2013ZX09103002-008)

摘　　要：目的:考察乌芩健脑方对Aβ海马注射致痴呆模型大鼠海马细胞周期素依赖性蛋白激酶5(cyclin-dependent kinases-5,CDK5)和糖原合成激酶3(glycogen synthase kinase-3,GSK-3)表达的影响。方法:用聚集状态的Aβ25-35大鼠双侧海马注射建立AD模型,药物干预后,采用Morris水迷宫评价大鼠学习记忆能力,采用免疫组化的方法检测大鼠海马CDK和GSK-3的表达和平均光密度。结果:乌芩健脑方18.48g/kg、9.24 g/kg和4.63 g/kg三个剂量组能显著缩短逃跑潜伏期和第三象限时间,延长第一相限时间和平台停留时间的作用,与模型对照组比较有显著的统计学意义;乌芩健脑方18.48g/kg和9.24 g/kg剂量组海马区CDK5表达较模型对照组显著降低,18.48g/kg、9.24 g/kg和4.63 g/kg三个剂量海马GSK-3的表达均较模型对照组表达显著降低,有显著的统计学意义。结论:乌芩健脑方具有较好的改善痴呆大鼠模型学习记忆功能的作用,并能显著抑制海马区CDK5和GSK-3的异常高表达,提示其抗AD的作用机制可能与通过抑制异常高表达的cdk5和GSK-3,阻止tau蛋白的过度磷酸化有关。Objective： To explore effects of Wuqinjiannao Fang （ Radix Polygoni Multiflofi Praeparata cum Succo Glycines Sotae and Radix Scutel- laria Brain-strengthening Decoction） on expression of cyclin-dependent kinases-5 and glycogen synthase kinase-3 in Alzheimer disease （AD） rats. Methods： Rats were induced by injecting agglomerate Aβ25-35 into bilateral hippocampus. After medication intervention, the learning and memory ability of AD rats was evaluated by Morris water-maze, and expression and average optical density of CDK5 and GSK-3 in hippo- campus were tested by immunohistochemistry. Results： Compared with vehicle control group, the latent period of escaping and the third quadrant time of medication groups of Wu Qin Jian Nao Fang in dose of 18.48g/kg,9.24 g/kg and 4.63 g/kg were significantly shortened, and the first quadrant time and platform residence time were significantly prolonged （P 〈 0.05） ; CDK5 of groups in dose of 18.48g/kg and 9.24 g/kg were significantly decreased ; and GSK-3 of all medicated groups were significantly decreased （ P 〈 0.05 ）. Conclusion： Wuqin-jiannao Fang can improve learning and memory abilities of AD rats, and significantly inhibit expression of CDK5 and GSK-3, which presents that the decoration may anti-AD by inhibiting CDK5 and GSK-3 to prevent over phosphorylation of tan protein.

关 键 词：乌芩健脑方 阿尔茨海默病 CDK5 GSK-3 

分 类 号：R285.5[医药卫生—中药学]