谷氨酰胺和IGF-I对晋岚绒山羊卵母细胞体外成熟的影响  

Effect of Glutamine and IGF-I on the Oocyte maturation of Jinlan Cashmere Goats

作　　者：张春香[1] 秦雪[1] 郑亚琳[1] 张彩霞[1] 岳文斌[1] 任有蛇[1] 靳黎[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《中国草食动物科学》2014年第5期26-28,共3页China Herbivore Science

基　　金：山西省科技攻关计划项目(20110311029)

摘　　要：为了探讨在成熟培养液中添加谷氨酰胺和IGF-I对晋岚绒山羊母羔卵母细胞体外成熟的影响。随机选择12只6-8周龄晋岚绒山羊母羔为试验动物，用FSH进行超数排卵，活体采集卵母细胞，并对未成熟的卵母细胞进行体外成熟培养。在培养液中添加0、50、100和150μg/mL的谷氨酰胺及0、25、50和100 ng/mL的IGF-I。结果表明：培养液中谷氨酰胺添加量为100μg/mL时，能显著提高卵母细胞成熟率（P〈0.05）；在培养液中添加100μg/mL谷氨酰胺的基础上，再添加50 ng/mL的IGF-I可以进一步显著提高卵母细胞的成熟率（P〈0.01）。The objective of the study was to investigate the effect of glutamine and IGF-I on oocyte maturation in Jinlan Cashmere goats. Twelve 6-8 week-old Jinlan cashmere female kids were selected to superovulate by FSH injection. The immature oocytes were collected by ovum pick-up, and culture in vitro maturation with supplementation of different concentration of glutamine （0,50,100 and 150μg/mL）and IGF-I （0,25,50 and 100 ng/mL）in maturation media. The results showed that the maturation rate of oocytes could be significantly improved by adding 100μg/mL glutamine to the maturation media （P〈0.05）. After addition 100μg/mL glutamine,adding 50 ng/mL IGF-I could markedly increase the rate of occytes maturation（P〈0.01）.

关 键 词：晋岚绒山羊母羔 卵母细胞 体外成熟 谷氨酰胺 IGF-I 

分 类 号：S827.3[农业科学—畜牧学]