繁殖期雌雄同体虾夷扇贝生殖腺组织学观察  

Histological research on the gonad of hermaphrodite Patinopecten yessonesis in breeding period

作　　者：周丽青[1] 杨爱国[1] 王清印[1] 郑言鑫 刘志鸿[1] 吴彪[1] 孙秀俊[1] 郑利兵[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室,青岛266071

出　　处：《高技术通讯》2014年第8期874-880,共7页Chinese High Technology Letters

基　　金：国家科技基础条件平台(2007DKA30470);黄海水产研究所基本科研业务费(20603022012003);山东省自主创新专项(2013CXC80202)资助项目

摘　　要：历时3年,采用Bouin's液固定和常规组织切片技术,对照雌性和雄性生殖腺组织结构,研究了繁殖期雌雄同体生殖腺组织的结构特点及形成原因。结果显示:虾夷扇贝雌雄同体的存在与海区养殖环境有关,山东烟台养殖海区的雌雄同体比例高达19.2%。雌雄同体两性生殖腺滤泡发育不同步且间隔不分明,2-3月份部分雌性生殖腺中出现迅速增殖的雄性生殖细胞或滤泡,成为雌性为主的雌雄同体;4月份随着雌雄生殖腺滤泡的共同生长和部分排放,形成以雄性生殖腺为主或雌雄相间的雌雄同体。雌性转化为雄性是主要性逆转方向,229只虾夷扇贝中仅1例推测为雄性向雌性逆转,有些个体存在雌雄两种生殖细胞同时成熟和排放的现象,是否存在两种性别均衡发育有待进一步研究。A 3-year histological study on the gonad of hermaphrodite Patinopecten yessoensis in breeding period by using the techniques of Bouins solution fixing and general tissue slicing was performed according to the organizational structural characteristics of female and male gonads to investigate the cause of hermaphrodites formation.The study shows the existence of hermaphrodites is commonly associated with the aquaculture environment.The proportion of hermaphrodites is up to 19.2％ in Yantai marine culture area of Shandong Province.The development of hermaphrodite gonad folliculi is asynchronous and its separation is not clear.Rapidly proliferative male gametes or folliculis are found in female gonads from February to March every year,with the gametes growth together and partly releasing,while in April the gonad of hermaphrodite Patinopecten yessoensis is mainly male or intersexed.The sex reversal direction is mainly from female to male,having only one scallop in 229 samples reversing from male to female.In the gonads of a few hermaphrodites,the female and male grerms are both found mature and released synchronously.Whether both sexes develop evenly in hermaphrodite gonad or not is still in need of further study.

关 键 词：虾夷扇贝 雌雄同体 性逆转 生殖腺 卵子退化 组织学 

分 类 号：S917.4[农业科学—水产科学]