刺五加多糖对断奶仔猪外周血淋巴细胞信号传导的影响  

Effects of Acanthopanax senticosus Polysaccharide on Signal Transduction of Peripheral Lymphocyte of Weaning Piglets

作　　者：韩杰[1] 边连全[1] 刘显军[1] 张飞[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110866

出　　处：《天然产物研究与开发》2014年第10期1589-1592,1584,共5页Natural Product Research and Development

基　　金：国家"十二五"科技支撑计划项目(2011BAD28B01-02);科技部科技成果转化项目(2009GB2B000068);2014辽宁省博士科研启动基金资助项目(20141061)

摘　　要：为了初步探讨刺五加多糖(ASPS)调节仔猪免疫功能的作用机制,试验研究了ASPS对体外培养的仔猪外周血淋巴细胞信号传导的影响。试验取28日龄断奶仔猪的外周血制备淋巴细胞悬液,分离其中的T、B淋巴细胞,分别与培养体系终浓度为0、40、80、160、320μg/mL ASPS共同孵育,MTT法测定T、B淋巴细胞转化率;在培养体系中加入TLR4抗体初步探讨ASPS影响淋巴细胞的信号传导途径。结果表明,ASPS能显著促进体外培养的仔猪外周血T淋巴细胞转化率以及淋巴细胞IL-2的分泌量(P<0.05),而对B淋巴细胞转化率和IL-4水平无显著影响(P>0.05)。当培养体系中无TLR4抗体时,ASPS对淋巴细胞分泌TNF-α、NO、iNOS和NF-κB因子水平有显著影响(P<0.05),而当培养体系中加入TLR4抗体后,ASPS对上述指标无显著影响(P>0.05)。上述结果提示,T细胞可能是ASPS直接作用的靶细胞之一,ASPS调节淋巴细胞免疫功能可能是通过TLR4/NF-κB信号通路起作用。The experiment concerning in vitro effect of Acanthopanax senticosus polysaeeharide （ASPS） on signal trans- duetion of peripheral lymphocyte was conducted to investigate the mechanism of immunoregulation effect of ASPS. Pe- ripheral blood lymphocyte suspension was prepared, and T, B cells of which were separated and co-incubated with or without various concentrations of ASPS （40,80,160,320 μg/mL） separately to determine lymphocyte preliferation measured by MTT assay. Anti-TLR4 was designed to discuss the lymphocyte receptor. The results showed that ASPS sig- nifieantly improved the T cell proliferation and the concentration of IL-2 secreted by lymphocyte （ P 〈 0.05）, but had no significant effect on the B cell proliferation and the concentration of IL-4 （ P 〉 0.05）. ASPS induced the production of TNF-α, NO, iNOS as well as NF-KB secreted by lymphocyte when TLR4 antibody was not supplemented in culture solu- tion （P 〈 0.05 ）, and these indices were not affected when TLR4 antibody was added in the culture solution （P 〉 0. 05）. These results suggested that T lymphocyte probably as one of the direct target ceiLs was mediated by ASPS through TLR4/NF-κB signaling pathway.

关 键 词：刺五加多糖 淋巴细胞增殖 信号分子 仔猪 

分 类 号：R828.9[医药卫生—临床医学]