毛脉酸模内生真菌蒽醌类次生代谢产物的筛选及含量分析  

Screening and Determination of Anthraquinones Secondary Metabolites from Endophytic Fungi of Rumex gmelini Turcz.

作　　者：郭美[1] 王谦博 王宗伟[1] 贾力维[1] 王振月[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]黑龙江省食品药品检验检测所,哈尔滨150010

出　　处：《天然产物研究与开发》2014年第10期1634-1637,共4页Natural Product Research and Development

基　　金：国家自然科学基金项目(30970300);哈尔滨科技创新人才研究专项基金项目(2010rfxxs031)

摘　　要：本文通过对毛脉酸模内生真菌的发酵培养得到其次生代谢产物,采用HPLC法对内生真菌中蒽醌类成分进行筛选。以大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素五种蒽醌类成分为参照,筛选出3株真菌含大黄素、3株真菌含大黄酸、5株真菌含芦荟大黄素,其中含量最高为683μg/g、最低为8μg/g。将各菌株所含成分与各菌株分离部位的相应成分进行比较,相对宿主植物而言各菌株均有较高的含量。本实验分析方法准确、快捷,适用于毛脉酸模内生真菌的代谢产物研究。In this paper, the secondary metabolites of anthraqulnones were obtained by fermentation of endophytic fungi of Rumex gmelini Turcz.. HPLC was used to falter different anthraquinanes from endophytic fungi of R. gmelini. With 5 an- thraquinone compounds, including emodin, rhein, chrysophanol, physcion and aloe-emodin as reference, the endophytic fungi with the secondary metabolites of anthraqulnones were screened out,in which 3 strains were screened out to contain emodin,3 strains contained rhein, and 5 strains contained aloe-emadin. Among these fungi, the highest and lowest yield of anthraquinones was 683 p,g/g and 8 p.g/g, respectively. The strains had higher concentrations of anthraquinones be- tween each strains and its respective separated parts in R. gmelini. The developed HPLC method was accurate and fast, and it was applicable to analysis metabolites of endophytic fungi from R. gmelini.

关 键 词：毛脉酸模 内生真菌 HPLC 蒽醌类次生代谢产物 

分 类 号：Q946.91[生物学—植物学]